Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that had already been in formailin two weeks (was FFPE). Normally would do on a frozen 1:40? ....We have background everywhwere on first run...suggestions? Neg is also shining??? Crazy researcher's! Got to love them...when will they learn to ask first?
We are diluting primary in plan two, and will stain over-night at 4 degrees....but, this thing is lit-up like the SUN! I was expecting low signal? Go figure? -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Sunday, May 22, 2011 1:01 PM To: [email protected] Subject: Histonet Digest, Vol 90, Issue 27 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. to Rene J Buesa (Maxim Peshkov) ---------------------------------------------------------------------- Message: 1 Date: Sun, 22 May 2011 13:43:07 +0400 From: Maxim Peshkov <[email protected]> Subject: [Histonet] to Rene J Buesa To: Rene J Buesa <[email protected]> Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=windows-1251 I am sorry, that uses histonet for personal goals. Dear Rene! Last 3 my answers for you are returned back to me. Your e-mails I got successfully. Support service of mail.ru wrote that problem is at yahoo.com server. Please check your postmaster service. Here is my post-address: Maxim Peshkov, ul. Mikhailovskaya, 90-A, 347909, Taganrog, Russia. Sincerely, Maxim. mailto:[email protected] ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
