Have you checked the pH in the coplin jars? Perhaps someone has labelled them wrong? Or is there any dilution-effect while staining, that raises the pH? I think about covering tapwater-wet slides with little amount of dye-solution? Or the acetic acid is too old, so the right pH isn't reached from the beginning?
Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von mohamed abd el razik Gesendet: Samstag, 04. Juni 2011 18:00 An: [email protected] Betreff: [Histonet] why no replay???? thanx to all kind replays from sgoebel, Paula Pierce and JR R. but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is: i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida and these was confirmed _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
