I concur on this. If my boss will let me, I can send you our elaborate protocol for H&E staining, It involves many dishes and many washes. But your H&E will be perfect. I don't have the protocol with me right now,as I'm at home but tomorrow I will ask about sending you the protocol. I don't see why we couldn't send you ours, but I'm going to ask just in case.
A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 16, 2011 at 2:53 PM, Rathborne, Toni < [email protected]> wrote: > Our protocol is to: > > Use a positively charged slide > Fix in 10% formalin for 1 minute > Rinse in tap (a few dips) > Gill 3 for 1 minute > Rinse in tap (a few dips) > 3-5 dips in bluing > Rinse in tap (a few dips) > Eosin 2-3 dips > 100% ETOH (a few dips) > 100% ETOH (a few dips) > Xylene (dip until clear) > Mount with Richard- Allan Mounting Medium > > After the Gill3, Eosin, and last alcohol, the back of the slide is wiped > clean of excess solution to prevent carryover. > > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Reynolds,Donna M > Sent: Tuesday, August 16, 2011 10:13 AM > To: '[email protected]' > Subject: [Histonet] H&E on Frozen > > We do this frequently. > Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes. > Wash several changes of tap water > Stain with Harris Hematoxylin for 3 minutes Proceed as you would for > paraffin sections Get great staining. > I am sure if you are using a different Hematoxylin you could use it the > same way you use it for paraffin > > Donna Reynolds HT(ASCP) > Chief Histology Technician, Core Immuno Lab U. T. M.D. Anderson Cancer > Center, Houston, TX > 713-792-8106 > e-mail [email protected] > > Message: 2 > Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT) > From: Jennifer Sipes <[email protected]> > Subject: [Histonet] H&E for Frozen Tissue > To: "[email protected]" > <[email protected]> > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=iso-8859-1 > > Does anyone have a good protocol for staining frozen sections with H&E?? > Also, any mounting medium suggestions?? This is the first time I've been > asked to do this for frozen. > ? > Thanks a bunch everyone! > Jen > ? > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD? 21205 > phone:???? 410-614-0131 > fax:???????? 410-955-9677 > cell:???????? 443-631-6361 > e-mail:? [email protected] > > ------------------------------ > > ** > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
