I think I've found something. Obviously the staining result is sensitive for special coverslipping medium. Patient slide was coverslipped with Pertex, control slide was coverslipped with Eukit. Interesting Gudrun
-----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Gudrun Lang Gesendet: Donnerstag, 15. September 2011 17:17 An: [email protected] Betreff: [Histonet] warthin starry fading Hi all! Today I stained Warthin Starry for Hp. This time I was lucky to have the first time purchased control tissue (Spirochetes control from Sigma) for comparison. In the package there was also a ready stained slide with really nice black spirochetes in yellow-brownish tissue/nuclei. This was stained with a Sigma Steiner Kit, but I thought my Warthin Starry should look the same. So, after staining, the patient tissue shows nice Hp in yellow/brownish background, but nuclei didn't stain. The control tissue had black-sprinkled nuclei and no spirochetes at all. I was a little bit disappointed, but at least the patient tissue was ok for diagnosis. A few hours later I looked at the control slide again and found, that the whole black staining had faded till a few speckles. (Patient slide was out of reach to look at) Does anybody know why this fading did happen? I have no information about the fixation and processing of the control slides, but thought it must fit for FFPET. The only point is, that we received the slides several months before. Is aging a factor? Staining protocol in brief: Everything diluted in pH 4 water, made with citric acid. 30 min 1% AgNO3 5-7 min developer (2% AgNO3+gelatine+Hydrochinon) Waterrinse Dehydration, coverslipping Gudrun _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
