Was it decalcifying a tissue fixed in a formalin based fixative using a hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan.
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jennifer MacDonald Sent: 04 October 2011 12:47 To: Patsy Ruegg Cc: [email protected]; [email protected] Subject: RE: [Histonet] decal question Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" <[email protected]> Sent by: [email protected] 10/03/2011 11:27 AM To "'Sarah Dysart'" <[email protected]>, <[email protected]> cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email [email protected] web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:[email protected]] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; [email protected] Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: [email protected] Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:[email protected]> [email protected] web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
