Hello, For DNA, we use a new blande and we wipe down microtome, handles, front of blade holder with 95% ethanol on a gauze sponge. For RNA, use a new blade and wipe down all with RNAzap including the water bath and rinse with DEPC water. Use clean water in bath, distilled will work or DEPC water. Depending on the stringency of the assay. You can sometimes get away with a little less. But some of the assays that are being run can cost up to $1,000.00. So the time spent cleaning up is justified. Make sure you are wearing gloves for both RNA and DNA, during the whole process, even when handling the slides .(including the process of labeling slides). I usually lay the slides out on pape rtowels on the lab bench while labeling them.
Helen Fedor Johns Hopkins University TMA and Prostate Spore Lab Manager JHU SOM Uro/Pathology 600 N. Wolfe Street, Marburg Room 406 Baltimore MD, 21287-7065 410-614-1660 / Fax 410-614-3695 Pager 410-283-3419 ________________________________________ From: [email protected] [[email protected]] on behalf of Crowell, Thomas [[email protected]] Sent: Tuesday, October 04, 2011 12:44 PM To: [email protected] Subject: [Histonet] Paraffin sections for molecular assays Dear Histonetters, Can you tell me what procedures you use to prevent DNA/RNA contamination of tissue sections when handling multiple samples. Do you just wipe down blade holder and blade (or use a new blade) between samples, or do you use more stringent cleaning? Thomas Crowell Diagnostic Development Novartis Molecular Diagnostics 45 Sidney Street Cambridge, MA 02139 USA Phone +1 617 8717460 Fax +1 N.A. [email protected]<mailto:[email protected]> www.novartis.com<http://www.novartis.com/> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
