I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts. Thanks again =)
Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
