It may be specific labeling of "extra-follicular dendritic cells" that contain low molecular weight cytokeratin and, if your IHC assay is truly optimized, you won't be able to get rid of it. I use the LMW CK mAb clone 5D3 that labels CK8/18 and I see this reactivity all the time. I like it because it serves as an internal positive control.
Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Joanne Clark <[email protected]> 2/28/2012 11:27 AM >>> Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
