The ultra view platform changed its clearing process in November. After staining slides are to be run through two changes of soapy water and then distilled water. After that is done they go on a slide dryer/warmer for at least 15 minutes before going into xylene and then coverslipped. Our sales rep made me aware of the change when it happened. I opened a new kit yesterday and what I just wrote is what the package insert said to do.
Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: [email protected] [[email protected]] On Behalf Of anita dudley [[email protected]] Sent: Tuesday, March 13, 2012 4:49 PM To: [email protected] Subject: [Histonet] ventana uv alkaline phosphatase red for those of you that use the ultraview alk phos red on their machines, do you run the slides through alcohols? we are having a lot of trouble with the staining, sometimes it is good others no staining at all on our mart 1s or pin4 prostate bxs. I was told that we should not put them in alc. air dry after water or oven dry? just wondering is that how others are handleing their slides stained with the red kit? we have been running them down through the alc. with the dab ones. thanks so much anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
