Try Microwaving blocks in 10% NBF or alcoholic formalin for 2 hours at 45oC.
Works a treat with placentas Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Mandy O'Connor Sent: Tuesday, 20 March 2012 2:37 AM To: '[email protected]' Subject: [Histonet] Fixation for breasts Hey fellow histo nuts! Anyone have any good recommendations for fixing breast tissue? We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results. We usually end up re-processing the block again just to get a good slide that is semi readable. Thanks for any and all help! Mandy O'Connor HTL(ASCP) Yellowstone Pathology Instit. Billings, MT _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
