Hi All in Histoland,

Problem solving never ends! I am working up two antibodies for neutrophils 
which are NIMP-R14 from Abcam which it is a rat monoclonal and also 
Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have 
tried different conditions without any luck and was wondering if anyone has 
worked with these antibodies either from Abcam or another supplier and might 
have working conditions that have given results in the past. We are trying this 
on FFPE sections which the antibodies say they work on but I am thinking that I 
may try frozens. I was trying to avoid this however because I am using these 
antibodies on mouse WAT. Any help would be appreciated. Thanks!!!

Happy Monday morning!!!
Kind Regards!
John J Shelley

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