Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy
Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail [email protected] -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 12:25 PM To: [email protected] Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
