Beautiful reply! 

Sent from my iPhone

On May 29, 2012, at 4:58 PM, Cheryl <[email protected]> wrote:

> Hi Ann-
>  
> May we assume you've confirmed this is happening at embedding and have ruled 
> out any floaters happening during cutting?
>  
> When embedding, keep Kimwipes or other tissues around, keep the wells closed 
> and only pull out enough cassettes that you can keeep clean and clear of your 
> working area.  Wipe forceps and surfaces between blocks containing fragmented 
> or friable tissue, don't put forceps back in the wells without wiping. You 
> can stack a few guaze pads on top of the spout to wipe as you replace the 
> forceps and change the pads frequently.   Always, always only open one 
> cassettte at a time and never leave the station with an open cassette on the 
> station.  Finish before standing or recap and replace.  
>  
> If you are working with currettes, cell blocks, or other cellular, friable 
> tissue, open and unwrap on a clean surface (hot or cold - embedding station 
> surface or wipe or l'absorb) and don't reuse the surface before wiping or 
> replacing.  If you're using knives or scalpels to scrape, make sure the 
> handles and connection points aren't harboring residual tissue. Buy those 
> little seamless paring knive from the dollar store--they fit in the wells and 
> wipe easily.  Use swabs between embedding sessions or between people trading 
> places to clean the wells and then clean them again at the end of the 
> embedding session.  
>  
> If you keep molten paraffin in the hold bins, filter or replace frequently 
> and do not reuse.  If you keep the hold bins dry, clean routinely (daily)  
> Clean your molten wax chamber periodically to remove contaminants and keep 
> the filter from clogging over time. Most embedding station mfc don't condone 
> running xylene through the tubes & pumps--clean hot wax will do the job.
>  
> We always make it the responsibility of each person to clean both at the end 
> of embedding AND to clean again before starting to fully assure the wells and 
> surfaces were clear and eliminate a possible cross if one person in the chain 
> forgets...double system processes like you double check specimen IDs. 
>  
> You'll go through a whole bunch of kimwipes--but they are much cheaper than 
> gauze and SOOO much better than a cross contamination situation.  IF it still 
> happens, it's time to track who embeds each block to see if there's a pattern 
> by person.  The point is not to write people up but to support developing 
> clean habits and to adjust their habits to do it to their best ability.  
>  
> Wipe wipe wipe wipe wipe!!  Hope this helps!
> 
> Cheryl Kerry, HT(ASCP) 
> Full Staff Inc. 
> Staffing the AP Lab by helping one GREAT Tech at a time.  
> 281.852.9457 Office
> 800.756.3309 Phone & Fax 
> [email protected] 
> 
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