We also use safranin on all our small biopsies and also our breast biopsies with no adverse effects to any of our IHC or ISH. Cindy
Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Wednesday, June 20, 2012 10:32 AM To: histonet@lists.utsouthwestern.edu; Carol Freeman Subject: Re: [Histonet] Eosin staining for small biopsies We use safranin (used in Microbiology as a counterstain) on our small biopsies. We apply a small drop during grossing. It does not affect staining of H&E, IHC or ISH. We like this because it is an intense red that doesn't leach out in the alcohols of processor. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com >>> "Freeman, Carol" <carol.free...@utoledo.edu> 6/20/2012 8:46 AM >>> Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet