We make up a "marking Eosin" that we use on small bx before we place them in cassettes.
We do not use actual teabags, but the mesh bags from Thermo Fisher that are not hard to pull apart and we never have anything sticking I the corners. I do know what you are referring to as some of the mesh bags ot teabags on the market are not mad of as fine material and are hard to pull apart and allow for specimens to gather in the corners. Our mesh bags are used for all specimens the PA's can "pour" into the bags. We also use the Obex papers for needle bx type specimens that can be laid out nicely on the paper and folded over the tissue once for optimal processing. On tissue processors, we are new this year to the Peloris processor from Leica and love the versatility of two retorts and the great reagent management which allows for less waste and less maintenance tech time. I was always a Sakura VIP fan previous, still am, but cannot compare the two retort option and how LEAN it has mad our lab! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 08, 2012 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: opinion on heating slides prior to IHC (Teri Johnson) 2. Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? (Jennifer Johnson) 3. Re: Teabags (Bob Richmond) 4. RE: Re: Teabags (Sarah Dysart) 5. RE: Re: Teabags (Clouse, Rosanna) 6. Re: Re: Teabags (Paula Pierce) 7. RE: Re: Teabags (Shirley A. Powell) 8. tissue highlighting for visibility (cont...@histocare.com) 9. decalcification of premolar teeth (dog) (Alice Fraser) 10. National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! (Jack Ratliff) 11. help ! paraffin section (Megha Kumar) 12. Re: tissue highlighting for visibility (Lee & Peggy Wenk) 13. Re: Re: Teabags (Lee & Peggy Wenk) 14. RE: tissue highlighting for visibility (MaryK Mendell) 15. Tissue Processor (Heckford, Karen - SMMC-SF) 16. RE: tissue highlighting for visibility (Vanessa Perez) 17. Re: Tissue Processor (Rene J Buesa) 18. Re: tissue highlighting for visibility (Rene J Buesa) 19. RE: decalcification of premolar teeth (dog) (Rittman, Barry R) 20. Re: help ! paraffin section (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Aug 2012 17:30:04 +0000 From: Teri Johnson <tjohn...@gnf.org> Subject: [Histonet] Re: opinion on heating slides prior to IHC To: "jefthomp...@salud.unm.edu" <jefthomp...@salud.unm.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9f3cfee76e51b64991c7485270890b400cdcb...@ex4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to say they might have had to try multiple antibodies to find one that would work under those conditions? The only way to know if heat or other conditions will negatively affect your target protein is to test it empirically. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 2 Date: Tue, 7 Aug 2012 12:11:53 -0700 From: Jennifer Johnson <jjohn...@scripps.edu> Subject: [Histonet] Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9b9f464463cb36488e8904cf8336917fb76bb57...@exch-ccr01.lj.ad.scripps.edu> Content-Type: text/plain; charset="us-ascii" Hi Guys Does anyone have a copy of the manual for the Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? Thanks in advance. Jennifer L. Johnson, Ph.D. Staff Scientist The Scripps Research Institute 10550 North Torrey Pines Road La Jolla, CA 92037 jjohn...@scripps.edu ------------------------------ Message: 3 Date: Tue, 7 Aug 2012 15:22:57 -0400 From: Bob Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Teabags To: histonet@lists.utsouthwestern.edu Message-ID: <caoksrh7eiyqof1f8dk9ssww+vny-u-czzve+ohu68mmk_pk...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ------------------------------ Message: 4 Date: Tue, 7 Aug 2012 19:27:10 +0000 From: Sarah Dysart <sdys...@mirnarx.com> Subject: RE: [Histonet] Re: Teabags To: Bob Richmond <rsrichm...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8a70a9b2ecdd084dacfe6c59fcf86d5018d4d...@bl2prd0710mb363.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 7 Aug 2012 15:31:36 -0400 From: "Clouse, Rosanna" <rclo...@wellspan.org> Subject: RE: [Histonet] Re: Teabags To: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Message-ID: <9e4560b21d59954a9931ff35b7bccdd4021a444...@exch01.wellspan.org> Content-Type: text/plain; charset="us-ascii" For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclo...@wellspan.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ ------------------------------ Message: 6 Date: Tue, 7 Aug 2012 12:33:43 -0700 (PDT) From: Paula Pierce <cont...@excaliburpathology.com> Subject: Re: [Histonet] Re: Teabags To: Sarah Dysart <sdys...@mirnarx.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1344368023.96408.yahoomail...@web5706.biz.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart <sdys...@mirnarx.com> To: Bob Richmond <rsrichm...@gmail.com>; "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 7 Aug 2012 16:04:53 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: RE: [Histonet] Re: Teabags To: Paula Pierce <cont...@excaliburpathology.com>, Sarah Dysart <sdys...@mirnarx.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE24B4555B779@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" Same here. sp -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, August 07, 2012 3:34 PM To: Sarah Dysart; Histonet Subject: Re: [Histonet] Re: Teabags Ditto! ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Sarah Dysart <sdys...@mirnarx.com> To: Bob Richmond <rsrichm...@gmail.com>; "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, August 7, 2012 2:27 PM Subject: RE: [Histonet] Re: Teabags I use hair perm papers that I buy from a local beauty supply store.? WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas? 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 07 Aug 2012 18:10:25 -0400 From: cont...@histocare.com Subject: [Histonet] tissue highlighting for visibility To: Histonet@lists.utsouthwestern.edu Message-ID: <20120807181025.46opnxy2fok40...@mail.histocare.com> Content-Type: text/plain; charset=UTF-8 Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ??What do some of you guys do? www.HistoCare.com Histology Staffing ------------------------------ Message: 9 Date: Wed, 8 Aug 2012 13:56:08 +1200 From: Alice Fraser <toxpat...@gmail.com> Subject: [Histonet] decalcification of premolar teeth (dog) To: histonet@lists.utsouthwestern.edu Message-ID: <CAO8o5+w0isL=bhhlghd+g7ym94eh8sddysx909jqyk6w79k...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice ------------------------------ Message: 10 Date: Tue, 7 Aug 2012 23:12:35 -0400 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: [Histonet] National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu167-w653253ff1a9a2d3fc99cbcae...@phx.gbl> Content-Type: text/plain; charset="Windows-1252" Greetings! The Hard Tissue Committee of the National Society for Histotechnology would like to remind everyone that we are hosting a singe day Hard Tissue Forum event at the Doubletree by Hiltion in Bethesda, MD on August 18th! You won't want to miss out on this opportunity to learn about and witness via "LIVE" demonstration all current forms of resin/plastics histology equipment. A special treat this year will be a first ever showing and demonstration in North America of a newly developed non-contact laser microtome (TissueSurgeon) to cut micron thin sections of a variety of tissue types! 7:30am - Registration Opens 8:00am ? Hard Tissue Histology: An Historical And Current Perspective of Microtomy Technique ? Jack L Ratliff 9:00am ? Skeletal Analysis With MicroCT: What You Need To Know And Why You Need To Know It ? Daniel S Perrien, Ph.D. 10:30am ? Refreshment Break 10:45am ? Bone, Biomaterials And Bugs: Resin Microtomy And The Rotary Microtome ? Damien Laudier, BS, HTL(ASCP), QIHC 12:15pm ? Lunch On Your Own 1:15pm ? Ground Section Microtomy Techniques (Parts I & II): Diamond Materials Combined With Manual And Semi-Automated Grinding Methods To Collect And Polish A Variety Of Resin Embedded Specimens ? (PART I) Joe Tabeling & Jack L Ratliff, BA; (PART II) Linda Durbin & Robert A Skinner 2:45pm ? Refreshment Break 3:00pm ? New Results In Laser Sectioning For General Histology, Tissue Engineering, Medical Device Implants And Industrial Analyses ? Heiko Richter, Ph.D. 4:00pm ? Histomorphometry of Bone: A Quantitative Description of Bone Histology Using Sub-Micron Resolution Optical Microscopy ? Nathanael H Reveal You can still register online at https://s3.goeshow.com/nsh/2012HT/ereg572259.cfm?pg=register or show up at the program and register onsite at 7:30am before the start of the meeting. We hope to see you all there on the 18th! Best Regards, Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology ------------------------------ Message: 11 Date: Wed, 8 Aug 2012 09:15:47 +0530 From: Megha Kumar <meg...@g.clemson.edu> Subject: [Histonet] help ! paraffin section To: histonet@lists.utsouthwestern.edu Message-ID: <cagnji_wt9gfd08ghku72m5igvmv+n0_4ocsth6yhjag65hr...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ------------------------------ Message: 12 Date: Wed, 8 Aug 2012 05:33:45 -0400 From: "Lee & Peggy Wenk" <lpw...@sbcglobal.net> Subject: Re: [Histonet] tissue highlighting for visibility To: <cont...@histocare.com>, <Histonet@lists.utsouthwestern.edu> Message-ID: <75FEA5CFE2B246ED8F5B9D00FD2282A9@HP2010> Content-Type: text/plain; format=flowed; charset="utf-8"; reply-type=original Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 8 Aug 2012 05:36:19 -0400 From: "Lee & Peggy Wenk" <lpw...@sbcglobal.net> Subject: Re: [Histonet] Re: Teabags To: "Clouse, Rosanna" <rclo...@wellspan.org>, <Histonet@lists.utsouthwestern.edu> Message-ID: <BFA4DEBDB1CA47F993F82674F3E6AF11@HP2010> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hint when using these - do NOT try to fold them up into a nice looking square. Once processed and in paraffin, it is very difficult to find the edge, to try to open back up. Fold into a not nice to look at, off-set square that is slightly crumpled. Much easier to find the edge. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -----Original Message----- From: Clouse, Rosanna Sent: Tuesday, August 07, 2012 3:31 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Teabags For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5" x 4" sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclo...@wellspan.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes >>I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue.<< I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 8 Aug 2012 06:47:56 -0400 From: MaryK Mendell <kmend...@goldbergmd.net> Subject: RE: [Histonet] tissue highlighting for visibility To: Lee & Peggy Wenk <lpw...@sbcglobal.net>, "cont...@histocare.com" <cont...@histocare.com>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <96138C8AB728814B9A576E4364EF84F9012BD954D328@EXMBX01.mmeprod.cbeyond> Content-Type: text/plain; charset="us-ascii" ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpw...@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 8 Aug 2012 05:11:13 -0700 From: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> Subject: [Histonet] Tissue Processor To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <ccc690f0f65f9348bbbf341dff0d175a19e563b...@phx-msg-007-n1.chw.edu> Content-Type: text/plain; charset="us-ascii" I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 16 Date: Wed, 8 Aug 2012 07:53:24 -0500 From: Vanessa Perez <vpe...@pathreflab.com> Subject: RE: [Histonet] tissue highlighting for visibility To: MaryK Mendell <kmend...@goldbergmd.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c80b5ee351aad74dadab70fabcb78d193bca8da...@exchange.pasa.local> Content-Type: text/plain; charset="us-ascii" We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropyl....this also helps in keeping techs from accidently using isopropyl as absolute or vice-versa... when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MaryK Mendell Sent: Wednesday, August 08, 2012 5:48 AM To: Lee & Peggy Wenk; cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue highlighting for visibility ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk [lpw...@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -----Original Message----- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 8 Aug 2012 07:25:22 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Tissue Processor To: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1344435922.89103.yahoomail...@web121405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sakura Ren? J. ________________________________ From: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, August 8, 2012 8:11 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down.? What tissue processor would you buy and why?? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 8 Aug 2012 07:32:39 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] tissue highlighting for visibility To: "cont...@histocare.com" <cont...@histocare.com>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <1344436359.627.yahoomail...@web121402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make the solution a "pale pink". That amount is enough to give a faint "pink hue" to the tissue to ease its localization. This does not interfere with any stain done after wards. Ren? J. ________________________________ From: "cont...@histocare.com" <cont...@histocare.com> To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 6:10 PM Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. ?What do some of you guys do? http://www.histocare.com/ Histology Staffing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 8 Aug 2012 09:29:29 -0500 From: "Rittman, Barry R" <barry.r.ritt...@uth.tmc.edu> Subject: RE: [Histonet] decalcification of premolar teeth (dog) To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <12a4dafc2febb84b8ded5f5e9201b4e917c1388...@uthcms1.uthouston.edu> Content-Type: text/plain; charset="us-ascii" Alice I would recommend using sodium formate/formic acid mixture for demineralization as this is more gentle than most agents. I would not use hydrochloric acid unless you are shipwrecked on a desert island and that is the only chemical available to you. I am assuming that EDTA demineralization is not an option for you? Barry ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alice Fraser [toxpat...@gmail.com] Sent: Tuesday, August 07, 2012 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of premolar teeth (dog) Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 8 Aug 2012 07:48:32 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] help ! paraffin section To: Megha Kumar <meg...@g.clemson.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1344437312.51180.yahoomail...@web121405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What you describe is a typical example of poor paraffin infiltration = the paraffin has not infiltrated the tissue and when you prepare the final block it will consist of 2 different components; the tissue and the paraffin. That is why you end with a "good paraffin section" without the tissue. Poor paraffin infiltration is always caused by an improper sequence while tissue processing. Either the fixation is incomplete OR the dehydration is incomplete and there is water in the tissue when you go to the "clearing" stage OR the clearing stage is incomplete and the tissue still has alcohol (immiscible with paraffin) when the tissue goes to the paraffin OR the paraffin infiltration is too short. The problem resides in your processing protocol and there is nothing you can do about that?at the end. Try to check your processing protocol to eliminate the problem. If this is happening "all of the sudden" while you used to have good results previously, then you either have changed reagents or the reagents?are not in a good condition. Ren? J.? ________________________________ From: Megha Kumar <meg...@g.clemson.edu> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 11:45 PM Subject: [Histonet] help ! paraffin section Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 105, Issue 9 **************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet