Ah, I knew it. :( Thank you. Zoe
On Tuesday, November 13, 2012, Will Chappell wrote: > Nope, sorry. All your fat is dissolved. > > Sent from my iPhone > > On Nov 13, 2012, at 8:52 AM, z o n k e d <[email protected] <javascript:;>> > wrote: > > > Hello Histonetters, > > > > First time writer, long time reader. I'm a newbie tech in academia and I > > was given a simple task which I think I pretty much screwed up. > > > > I should have embedded half of a mouse liver in paraffin for microtome > > sectioning while the other half should have been embedded in OCT for > > cryosectioning (for oil red o). I made the mistake last night of placing > > both liver halves into the tissue processor. The liver I intended for OCT > > embedding is now hard as wax. Is there any way to deparaffinize processed > > organs and may I embed them in OCT for proper cryosectioning? I imagine > > that the liver would get dehydrated, I would get crappy sections, and Oil > > Red O won't work. > > > > Any suggestions are welcome. > > > > Thank you so much, > > > > Zoe W. > > > > > > -- > > "It costs nothing to say something kind. Even less to shut up > altogether." > > > > --Nathan Fillion > > _______________________________________________ > > Histonet mailing list > > [email protected] <javascript:;> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
