If you want to do TRAP staining for osteoclasts you will need to use 10 -15% EDTA at neutral pH to decalcify. It will take time but using agitation and changing the solution daily will facilitate the decalcification. Mike
Michael Starbuck, M.S. Laboratory Coordinator Bone Disease Program of Texas Histomorphometry Core GU Medical Oncology The University of Texas M. D. Anderson Cancer Center T7.3966 ph: 713 563-1212 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Vikrant Piprode Sent: Thursday, December 20, 2012 11:22 AM To: [email protected] Subject: [Histonet] (no subject) hi i want to know the best method to decalcify calvarial bone of mice for subsequent osteoclast staining and goldner's trichrome staining. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
