Please remove me from the mailing list. Thank you
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
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Sent: Friday, May 31, 2013 1:02 PM
To: [email protected]
Subject: Histonet Digest, Vol 114, Issue 30
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Contents of Histonet digest..."
Today's Topics:
1. Re: Processing thin slices of mouse cerebellum (Bob Richmond)
2. RE: Extra Money (Mitchell Jean A)
3. HT vs. HTL (McKenzie, Emily)
4. Re: antigen retrieval question (Jan Shivers)
5. Re: Extra Money (Lee & Peggy Wenk)
6. RE: Processing thin slices of mouse cerebellum (Hobbs, Carl)
7. Movats problem solved (Molinari, Betsy)
8. Steiner stain (Herring, Colleen)
9. Autotechnicon processor (margaret chadwell )
10. Re: Steiner stain (Rene J Buesa)
11. bone marrow aspirations (Diana McCaig)
12. RE: bone marrow aspirations (Hannen, Valerie)
13. Formalin (Scott, Allison D)
14. Re: Formalin (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Thu, 30 May 2013 13:27:02 -0400
From: Bob Richmond <[email protected]>
Subject: [Histonet] Re: Processing thin slices of mouse cerebellum
To: [email protected]
Message-ID:
<CAOKsRH7GTsju8e5X9K3=llpjt1fy6jqyx0uuw8va3gcrral...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Tom Truscott replies to Kathleen [Robert]: >>Several years and a couple
employers ago we attached small biopsies (for proper orientation) to small
squares of cucumber (which had been dehydrated in several changes of
alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded
together after processing and cut and stained as a unit with no adverse
effects.<<
I never saw it, but this cucumber technique was in fairly common use among
gynecologic pathologists 40 or 50 years ago, for embedding cervical biopsy
specimens. The cucumber in alcohol was provided to the GYN performing the
biopsy. I think I'd prefer a gin & tonic.
Bob Richmond
Samurai Pathologist
Maryville TN
------------------------------
Message: 2
Date: Thu, 30 May 2013 19:12:04 +0000
From: Mitchell Jean A <[email protected]>
Subject: RE: [Histonet] Extra Money
To: "'Morken, Timothy'" <[email protected]>, Sarah Dysart
<[email protected]>, "[email protected]"
<[email protected]>
Message-ID:
<16f90b93ca23d446980b3d591fd02dad05f...@uwhc-mbx14.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
To clarify: Winning NSH poster presentations in both Clinical and VIR
categories each receive $250.00 - but NSH does not reimburse any expenses for
poster presentation. Hope no one got their hopes up on this.... Jean Mitchell
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Morken, Timothy
Sent: Thursday, May 30, 2013 10:53 AM
To: joelle weaver; Sarah Dysart; Rene J Buesa; [email protected]
Subject: RE: [Histonet] Extra Money
Actually it could be like "earning" money because I believe a poster presenter
gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do
you think there are so many repeat presenters!?!?
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of joelle weaver
Sent: Thursday, May 30, 2013 8:37 AM
To: Sarah Dysart; Rene J Buesa; [email protected]
Subject: RE: [Histonet] Extra Money
Maybe to the nice folks at NSH :)
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: [email protected]
> To: [email protected]; [email protected];
> [email protected]
> Date: Wed, 29 May 2013 21:05:02 +0000
> Subject: RE: [Histonet] Extra Money
> CC:
>
> I plan on doing a poster for NSH...does that count =)
>
> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna
> Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
> From: Rene J Buesa [mailto:[email protected]]
> Sent: Wednesday, May 29, 2013 3:37 PM
> To: joelle weaver; Sarah Dysart; [email protected]
> Subject: Re: [Histonet] Extra Money
>
> Not even that! If you publish you will build a better c.v. but that is not
> assurance tof being appreciated by any prospective employer.
> In the rare event that it is appreciated you will be able to negotiate a
> better salary, but that is all!
> Ren? J.
>
> From: joelle weaver <[email protected]>
> To: Sarah Dysart <[email protected]>;
> "[email protected]"
> <[email protected]>
> Sent: Wednesday, May 29, 2013 4:31 PM
> Subject: RE: [Histonet] Extra Money
>
> Publish
>
>
>
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>
> > From: [email protected]<mailto:[email protected]>
> > To:
> > [email protected]<mailto:[email protected]
> > rn.edu>
> > Date: Wed, 29 May 2013 20:20:10 +0000
> > Subject: [Histonet] Extra Money
> >
> > Anyone know any ideas on how to make a couple extra bucks a month using our
> > histology-awesomeness??
> >
> >
> > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist
> > Mirna Therapeutics
> > 2150 Woodward Street
> > Suite 100
> > Austin, Texas 78744
> > (512)901-0900 ext. 6912
> >
> > _______________________________________________
> > Histonet mailing list
> > [email protected]<mailto:[email protected]
> > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> [email protected]<mailto:[email protected]
> .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> [email protected]
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
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------------------------------
Message: 3
Date: Thu, 30 May 2013 14:59:23 -0500
From: "McKenzie, Emily" <[email protected]>
Subject: [Histonet] HT vs. HTL
To: "'[email protected]'"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="us-ascii"
Can anyone provide job descriptions for HT vs. HTL. We are looking to separated
the two positions. If there are any suggestions please let me know.
Thank you,
Emily McKenzie
Memorial Medical Center
________________________________
This message (including any attachments) contains confidential information
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------------------------------
Message: 4
Date: Thu, 30 May 2013 15:08:33 -0500
From: Jan Shivers <[email protected]>
Subject: Re: [Histonet] antigen retrieval question
To: "Burton, Lynn" <[email protected]>
Cc: "[email protected]"
<[email protected]>
Message-ID:
<caeoc1q0l7a8ophed0t_rvnh8n424f0muq5bsz6gsvuheter...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Lynn, I use the Decloaker and also stain for BVD, so will get in contact with
you later this afternoon.
Jan Shivers
University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108
On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn <[email protected]>wrote:
> If anyone out there is using a decloaker for antigen retrieval, when
> doing ihc staining, would you please contact me on my e-mail address?
> I have been struggling with a BVD test for months. It is not working
> consistently and I want to explore using this method along with our
> Benchmark XT stainer from Ventana.
> Thank you,
> Lynn Burton
> Galesburg Animal Disease Lab
> Galesburg, Il
> [email protected]
> _______________________________________________
> Histonet mailing list
> [email protected]
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Thu, 30 May 2013 19:28:40 -0400
From: "Lee & Peggy Wenk" <[email protected]>
Subject: Re: [Histonet] Extra Money
To: "Morken, Timothy" <[email protected]>, "joelle weaver"
<[email protected]>, "Sarah Dysart" <[email protected]>,
"Rene J Buesa" <[email protected]>,
<[email protected]>
Message-ID: <A609F5305A164244B915E9314608F176@HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
The poster reimbursement has been addressed. So I'll talk about workshop
presenters. It's not a free trip, but some of it is paid. For presenting 1
workshop, you are reimbursed:
- air fare or cost of driving, whichever is least
- parking at airport (if flying) or at hotel (if driving)
- 2 nights of hotel (not to exceed the "convention" hotel rate). You pay for
the extra days of hotel you are there.
- registration fee ($35), but you have to pay for any workshops you want to
attend
- 1 banquet ticket
- meals for 2 days (I think it's about $35/day). If you know the cost of food
at convention hotels, $35 MIGHT cover part of breakfast and lunch. If you are
over the $35, you pay for the rest. If you are there more than 2 days, you pay
for your own food.
So presenting does not give you a free ride to the convention, but it does help
reduce the amount that you pay to attend.
If anyone is interested in presenting, it works best if you have presented
several times at your state meeting/symposium, and start building up a good
reputation as a good presenter. NSH usually does not ask a "first time
presenter" to talk at a national symposium. If you have no state organization,
ask NSH to help you start one. Or, start presenting at in-services where you
work. Don't have any of those? Start a monthly in-service, and put that on your
resume/CV. (yes, you will need a curriculum vitae to submit to NSH if accepted.)
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: Morken, Timothy
Sent: Thursday, May 30, 2013 11:52 AM
To: joelle weaver ; Sarah Dysart ; Rene J Buesa ;
[email protected]
Subject: RE: [Histonet] Extra Money
Actually it could be like "earning" money because I believe a poster presenter
gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do
you think there are so many repeat presenters!?!?
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of joelle weaver
Sent: Thursday, May 30, 2013 8:37 AM
To: Sarah Dysart; Rene J Buesa; [email protected]
Subject: RE: [Histonet] Extra Money
Maybe to the nice folks at NSH :)
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: [email protected]
> To: [email protected]; [email protected];
> [email protected]
> Date: Wed, 29 May 2013 21:05:02 +0000
> Subject: RE: [Histonet] Extra Money
> CC:
>
> I plan on doing a poster for NSH...does that count =)
>
> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna
> Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
> From: Rene J Buesa [mailto:[email protected]]
> Sent: Wednesday, May 29, 2013 3:37 PM
> To: joelle weaver; Sarah Dysart; [email protected]
> Subject: Re: [Histonet] Extra Money
>
> Not even that! If you publish you will build a better c.v. but that is
> not assurance tof being appreciated by any prospective employer.
> In the rare event that it is appreciated you will be able to negotiate
> a better salary, but that is all!
> Ren? J.
>
> From: joelle weaver <[email protected]>
> To: Sarah Dysart <[email protected]>;
> "[email protected]"
> <[email protected]>
> Sent: Wednesday, May 29, 2013 4:31 PM
> Subject: RE: [Histonet] Extra Money
>
> Publish
>
>
>
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>
> > From: [email protected]<mailto:[email protected]>
> > To:
> > [email protected]<mailto:[email protected]
> > rn.edu>
> > Date: Wed, 29 May 2013 20:20:10 +0000
> > Subject: [Histonet] Extra Money
> >
> > Anyone know any ideas on how to make a couple extra bucks a month
> > using our histology-awesomeness??
> >
> >
> > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist
> > Mirna Therapeutics
> > 2150 Woodward Street
> > Suite 100
> > Austin, Texas 78744
> > (512)901-0900 ext. 6912
> >
> > _______________________________________________
> > Histonet mailing list
> > [email protected]<mailto:[email protected]
> > rn.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> [email protected]<mailto:[email protected]
> .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> [email protected]
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
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------------------------------
Message: 6
Date: Fri, 31 May 2013 07:30:45 +0000
From: "Hobbs, Carl" <[email protected]>
Subject: RE: [Histonet] Processing thin slices of mouse cerebellum
To: "[email protected]"
<[email protected]>
Message-ID:
<00d6b8253eaed840b8d04e235497381831ff2...@am2prd0311mb399.eurprd03.prod.outlook.com>
Content-Type: text/plain; charset="iso-8859-1"
http://www.cellpath.us.com/magento/cellpath-on-line-shop/specimen-processing-and-embedding/small-biospy-processing/cellsafe/cellsafe-biopsy-capsule-blue.html
I find them very good for brain slices.
There is a deeper and a shallower space, depending on which way they are folded.
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson Centre for Age-Related Diseases
School of Biomedical Sciences
King's College London
Guys Campus
London SE1 1UL
?
Tel.020 7848 6810
fax 020 7848 6816
[email protected]
------------------------------
Message: 7
Date: Fri, 31 May 2013 11:00:07 +0000
From: "Molinari, Betsy" <[email protected]>
Subject: [Histonet] Movats problem solved
To: "[email protected]"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="utf-8"
Good Friday to all.
Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a
solution from another lab and voila, perfect staining. Thank you to everyone
for their suggestions. I will certainly keep them on file in the event this
ever happens again.
Have a good weekend.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates St.
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)
<http://www.texasheart.org>
Betsy Molinari
Senior Histology Research Technician
832-355-6524 | [email protected]<mailto:[email protected]> |
www.texasheart.org<http://www.texasheart.org>
6770 Bertner Ave., MC 1-283, Houston, TX 77030
[Texas Heart Institute][THI
News]<https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html>
[THI on Facebook] <http://www.facebook.com/Texas.Heart.Institute> [THI on
Flicker] <http://www.flickr.com/photos/texasheart/sets/> [THI on Google]
<https://plus.google.com/u/0/118043615690351997044/posts> [THI on Pinterest]
<http://pinterest.com/texasheartinst/> [THI on Twitter]
<http://twitter.com/Texas_Heart> [THI on You Tube]
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Message: 8
Date: Fri, 31 May 2013 07:16:49 -0400
From: "Herring, Colleen" <[email protected]>
Subject: [Histonet] Steiner stain
To: <[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
I am looking for some suggestions on a problem with our steiner stain. We have
a percipitate on the whole slide not just on the tissue and no matter what we
have done I can't seem to get rid of it. HELP!
________________________________________________________________________________________________________
The information in this communication is intended to be confidential to the
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the
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________________________________________________________________________________________________________
------------------------------
Message: 9
Date: Fri, 31 May 2013 11:27:58 +0000
From: "margaret chadwell " <[email protected]>
Subject: [Histonet] Autotechnicon processor
To: "[email protected] "
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-15"
Does anyone have an Autotechnicon Tissue Processor for sale?Thanks much!Margie
Chadwell
------------------------------
Message: 10
Date: Fri, 31 May 2013 07:11:29 -0700 (PDT)
From: Rene J Buesa <[email protected]>
Subject: Re: [Histonet] Steiner stain
To: "Herring, Colleen" <[email protected]>,
"[email protected]"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset=iso-8859-1
Usually any (and almost all) precipitates when using a silver stain is produced
by the silver solution reacting over some remnant of a previous solution not
completely removed.
Check your solutions, if needed prepare them anew and try again.
Ren? J.
From: "Herring, Colleen" <[email protected]>
To: [email protected]
Sent: Friday, May 31, 2013 7:16 AM
Subject: [Histonet] Steiner stain
I am looking for some suggestions on a problem with our steiner stain. We have
a percipitate on the whole slide not just on the tissue and no matter what we
have done I can't seem to get rid of it. HELP!
________________________________________________________________________________________________________
The information in this communication is intended to be confidential to the
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately
either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies.
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------------------------------
Message: 11
Date: Fri, 31 May 2013 15:00:57 +0000
From: Diana McCaig <[email protected]>
Subject: [Histonet] bone marrow aspirations
To: "'[email protected]'"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="us-ascii"
Can anyone share with me their process for processing bone marrow aspirations
and embedding them in paraffin blocks. Currently ours are being spread out
throughout the entire base mold and makes it cumbersome to screen. Has anyone
used histogel or making a button and embed that instead of just filtering the
aspirate and putting that is a cassette.
Thanks
Diana
------------------------------
Message: 12
Date: Fri, 31 May 2013 11:49:21 -0400
From: "Hannen, Valerie" <[email protected]>
Subject: [Histonet] RE: bone marrow aspirations
To: 'Diana McCaig' <[email protected]>,
"'[email protected]'"
<[email protected]>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB5E9@isexstore03>
Content-Type: text/plain; charset="us-ascii"
Diana,
We let the aspirates clot in a petri dish, cover them in formalin to fix the
clot and then make a block from the fixed clot. Process overnight in the
processor and embed the clot in the A.M. This process helps eliminate the
aspirate from spreading out in the base mold.
I hope this helps.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
[email protected]
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Diana McCaig
Sent: Friday, May 31, 2013 11:01 AM
To: '[email protected]'
Subject: [Histonet] bone marrow aspirations
Can anyone share with me their process for processing bone marrow aspirations
and embedding them in paraffin blocks. Currently ours are being spread out
throughout the entire base mold and makes it cumbersome to screen. Has anyone
used histogel or making a button and embed that instead of just filtering the
aspirate and putting that is a cassette.
Thanks
Diana
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------------------------------
Message: 13
Date: Fri, 31 May 2013 16:31:05 +0000
From: "Scott, Allison D" <[email protected]>
Subject: [Histonet] Formalin
To: "[email protected]"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset="us-ascii"
Hello to all in histoland. Happy Friday. Last week we were inspected by a new
group of surveyors that are like JCAHO called DNV. They said that we had to
much formalin stored in our gross room. We had 15 gallons. They told us that
we could only have 2 gallons there at a time. I have never heard of such. We
were just inspected by CAP and I might add that it was an intense inspection.
They checked everything. This never came up. What is the recommended amount of
formalin that can be stored in an area. Does it need to be in a flammable
cabinet? The cabinet that we have the formalin in is so old that we are
having a vendor to come out to check to see if it suitable for chemical storage.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287
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Message: 14
Date: Fri, 31 May 2013 09:41:17 -0700 (PDT)
From: Rene J Buesa <[email protected]>
Subject: Re: [Histonet] Formalin
To: "Scott, Allison D" <[email protected]>,
"[email protected]"
<[email protected]>
Message-ID:
<[email protected]>
Content-Type: text/plain; charset=iso-8859-1
Formalin is THEORETICALLY (and very UNLIKELY) "flammable, but putting it into a
flammables cabinet is going too far.
Try to find out those conflicting standards (JCAHO vs. CAP) and follow the
strictest.
Also please remember that many inspectors just "love to show off!" Some think
that have to "cite for something" because otherwise they are not doing a good
job, or do not know enough.?
Ren? J.
From: "Scott, Allison D" <[email protected]>
To: "[email protected]" <[email protected]>
Sent: Friday, May 31, 2013 12:31 PM
Subject: [Histonet] Formalin
Hello to all in histoland.? Happy Friday.? Last week we were inspected by a new
group of surveyors that are like JCAHO called DNV.? They said that we had to
much formalin stored in our gross room.? We had 15 gallons.? They told us that
we could only have 2 gallons there at a time.? I have never heard of such.? We
were just inspected by CAP and I might add that it was an? intense inspection.?
They checked everything. This never came up.? What is the recommended amount of
formalin that can be stored in an area.? Does it need to be in a flammable
cabinet?? The cabinet that we have the formalin in is so old that we are?
having a vendor to come out to check to see if it suitable for chemical storage.
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287
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representative of the intended recipient, you are hereby notified that any
review, dissemination or copying of this e-mail and its attachments is
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End of Histonet Digest, Vol 114, Issue 30
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