Hi All,
Has anyone tried to section cells cut from a trans well dish?
We have cells that are cultured and attach to a trans well membrane and our
pathologist wants to look at them on the membrane but the membrane is a thin
plastic film...see the problem.
We fixed the cells in the trans well for 5 minutes then cut the trans well out
of the well and bisected it and processed overnight we use paraffin type #1
paraffin. The next morning we embedded it with type #9 paraffin. The samples
cut but do not stay on the slide after xylene. I think the plastic does not
adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck
to the slide??
My pathologist does not want to change to a collagen membrane so he wants me
to find another (harder paraffin) to use, I don't think that will help.
Has anyone does similar processing/embedding?
I'll take any help I can get..
Thanks,
Jamie
Abbvie Bioreseach Center
Pharmacology
100 Research Dr.
Worcester,Ma 01605
OFFICE +1 508-688-3134
FAX +1 508-793-4895
EMAIL [email protected]<mailto:[email protected]>
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