Hi CJ,
we had this problem the year before last, it turned out to be the slides.
Apparently, the manufactor had a couple of "bad runs". We switched to Avantik
Biogroup Ultra Bond and haven't had a problem since. The bonus was they were
more reasonably priced compare to our "contract" vendor. Send me a e-mail if
you want their contact info.
Cassandra Davis
cda...@che-east.org
302-575-8095
Message: 11
Date: Mon, 5 Aug 2013 20:49:02 +0000
From: "Goins, Tresa" <tgo...@mt.gov>
Subject: [Histonet] RE: Slide dehydration After Staining Tissue
Falling Off
To: Christopher Jacobs <cjac...@clinpath.com>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<fa81e534f40def44a70f0fbbcd49d2e40c6d0...@doaisd5235.state.mt.ads>
Content-Type: text/plain; charset="us-ascii"
After the detergent treatment, rinse well in distilled water and dry in the
oven.
Dip in clearant and coverslip.
We do not treat our developed IHC slides in alcohols or clearant due to
potential loss of some labeling - not DAB.
Tresa
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher
Jacobs
Sent: Monday, August 05, 2013 11:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide dehydration After Staining Tissue Falling Off
Histonetters,
Recently my lab has been experiencing a significant occurrence of tissue
falling off our IHC slides. We are looking at a variety a factors and I have
been watching how some technicians dehydrate their slides after staining. We
are running Ventana IHC stainers, so we remove the oil based liquid coverslip
in a weak solution of Dawn and then run the slides down alcohols into xylene.
Some technicians are very aggressive in their agitation of the slides. It is
almost comical how much sloshing around in the reagents they do. How can I get
these people to ease up? Does anyone have any protocols on how many dips it
takes in each reagent to properly dehydrate slides?
Also, some of the other issues we are looking at causing tissue falling off the
slides are; tapping slides to remove water, not double-dipping slides, lotion
on hands, over temperature, and the brand of charged slides we are using. Can
anyone think of any other factors to look at?
Thanks!
"CJ" Christopher P. Jacobs, HT QIHC(ASCP)
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