Sorry I think that went out with me messing up spell check. Hit the button too fast. Anyway I am sure the point is there Dako - new - unit - better ability for IHC, ISH, CISH etc.
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Madeleine Huey Sent: Thursday, September 12, 2013 12:35 PM To: [email protected] Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24 O..o.oop! PC problem. Haley, I have experiences with all the above equipment's and here's my inf; 1) Leica H&E Autostainer + glass coverslipper - smaller size therefore use lesser counter space 2) Lesser cost $$ for Leica HE Autostainer / coverslipper 3) Bond Max/Bond III could do IHC & ISH simultaneously in one Autostainer, while DAKO IHC autostainer can do only IHC (no heating elements) 4) Bond can do DELAY start while you are sleeping & continuos flow as well, while DAKO need removed slides after AR & run immediately (batches) Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - IHC & Histology (Pathology) On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey <[email protected]>wrote: > From:"Huggins, Haley - MRMC" <[email protected]> > To: "[email protected]" > <[email protected] > > > Sent: Wednesday, September 11, 2013 4:46 PM > Subject: [Histonet] Equipment selection > > Hi all, > > I am trying to decide on what equipment to purchase. I have picked the > processor, microtomes, embedding centers and a few other pieces, but > now I still need to decide on the H&E Stainer and the immunostainer. > My options I have in front of me are for the H&E stainer are Sakura > Prisma stainer/film coverslipper and the Leica ST5020-CV5030 > stainer/glass coverslipper. For the Immunostainer, I am looking at the > Dako Autostainer and the Leica Bond Max. Any advice on any of these > instruments would be greatly appreciated. I am trying to finalize my > decision pretty quickly. Thank you in advance for any input. > > Haley, > I have experiences with all the above equipments and here's my inf; > 1) Leica H&E autostainer with glass coverslipper - smaller size (use > lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + > film coverslipper and smaller. > > > On Thu, Sep 12, 2013 at 10:02 AM, < > [email protected]> wrote: > >> Send Histonet mailing list submissions to >> [email protected] >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> [email protected] >> >> You can reach the person managing the list at >> [email protected] >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Equipment selection (Huggins, Haley - MRMC) >> 2. Re: Equipment selection (Rene J Buesa) >> 3. RE: Equipment selection (Huggins, Haley - MRMC) >> 4. RE: Equipment selection (Marcum, Pamela A) >> 5. Need your expertise (Webb, Dorothy L) >> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins) >> >> >> --------------------------------------------------------------------- >> - >> >> Message: 1 >> Date: Thu, 12 Sep 2013 08:55:39 -0700 >> From: "Huggins, Haley - MRMC" <[email protected]> >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa <[email protected]>, >> "[email protected]" >> <[email protected]> >> Message-ID: >> < >> 4f36ec93a5737d4f8a2974e8fb8e260615f5698...@phx-msg-007-n2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I >> might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:[email protected]] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; [email protected] >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> René J. >> >> From: "Huggins, Haley - MRMC" <[email protected]<mailto: >> [email protected]>> >> To: "[email protected]<mailto: >> [email protected]>" >> <[email protected] >> <mailto:[email protected]>> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected]<mailto: >> [email protected]> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT) >> From: Rene J Buesa <[email protected]> >> Subject: Re: [Histonet] Equipment selection >> To: "Huggins, Haley - MRMC" <[email protected]>, >> "[email protected]" >> <[email protected]> >> Message-ID: >> <[email protected]> >> Content-Type: text/plain; charset=iso-8859-1 >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> René J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" <[email protected]> >> To: Rene J Buesa <[email protected]>; "[email protected]" >> <[email protected]> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I >> might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:[email protected]] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; [email protected] >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> RenéJ. >> >> From:"Huggins, Haley - MRMC" <[email protected]> >> To: "[email protected]" < >> [email protected]> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 3 >> Date: Thu, 12 Sep 2013 09:10:47 -0700 >> From: "Huggins, Haley - MRMC" <[email protected]> >> Subject: RE: [Histonet] Equipment selection >> To: Rene J Buesa <[email protected]>, >> "[email protected]" >> <[email protected]> >> Message-ID: >> < >> 4f36ec93a5737d4f8a2974e8fb8e260615f5698...@phx-msg-007-n2.chw.edu> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Thanks, that is a good point. >> >> From: Rene J Buesa [mailto:[email protected]] >> Sent: Thursday, September 12, 2013 9:08 AM >> To: Huggins, Haley - MRMC; [email protected] >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> René J. >> >> From: "Huggins, Haley - MRMC" <[email protected]<mailto: >> [email protected]>> >> To: Rene J Buesa <[email protected]<mailto:[email protected]>>; " >> [email protected]<mailto: >> [email protected]>" >> <[email protected] >> <mailto:[email protected]>> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I >> might not be happy with in the future. >> >> Haley >> >> From: Rene J Buesa [mailto:[email protected]] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; [email protected]<mailto: >> [email protected]> >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> René J. >> >> From: "Huggins, Haley - MRMC" <[email protected]<mailto: >> [email protected]>> >> To: "[email protected]<mailto: >> [email protected]>" >> <[email protected] >> <mailto:[email protected]>> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected]<mailto: >> [email protected]> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Thu, 12 Sep 2013 16:30:13 +0000 >> From: "Marcum, Pamela A" <[email protected]> >> Subject: RE: [Histonet] Equipment selection >> To: "'Rene J Buesa'" <[email protected]>, "Huggins, Haley - MRMC" >> <[email protected]>, " >> [email protected]" >> <[email protected]> >> Message-ID: >> >> <[email protected]> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Actually the coverslipper from Leica is very good and can be linked >> to the stainer. It is also a glass coverslipper not tape. It is >> always about money and that may be the thing that decides no matter what any >> of prefers. >> >> -----Original Message----- >> From: [email protected] [mailto: >> [email protected]] On Behalf Of Rene J Buesa >> Sent: Thursday, September 12, 2013 11:08 AM >> To: Huggins, Haley - MRMC; [email protected] >> Subject: Re: [Histonet] Equipment selection >> >> If buying the Bond means that you are forced to "swallow" the Leica >> coverslipper go with Sakura and Dako. >> If they are making that "package" is because the Bond sells well but >> they are having to "swallow" the coverslipper. Price is important but >> in the long run it is better to increase productivity and avoid >> problems. The Sakura is much faster than the Leica coverslipper. >> René J. >> >> >> ________________________________ >> From: "Huggins, Haley - MRMC" <[email protected]> >> To: Rene J Buesa <[email protected]>; "[email protected]" >> <[email protected]> >> Sent: Thursday, September 12, 2013 11:55 AM >> Subject: RE: [Histonet] Equipment selection >> >> >> >> I actually know all the equipment. Well, I know 3 of the pieces >> really well, the Leica Bond Max is new to me. I did go to another lab >> to see it as well as the Leica stainer/coverslipper. I was impressed >> with the Bond Max, not as much with the coverslipper part of the >> stainer/coverslipper. The big issue I am having is if I want to go >> with the Bond Max, I technically need to go with the Leica >> stainer/coverslipper because it is lumped into a big package deal. If >> I want the Sakura Prisma stainer/film coverslipper, I go with the >> Dako immunostainer. I am having trouble deciding. It is boiling down >> to money, which sucks since I might be forced to go with something that I >> might not be happy with in the future. >> >> Haley >> >> From:Rene J Buesa [mailto:[email protected]] >> Sent: Thursday, September 12, 2013 5:32 AM >> To: Huggins, Haley - MRMC; [email protected] >> Subject: Re: [Histonet] Equipment selection >> >> I used the Sakura and the Dako, but it seems that the Leica Bond Max >> is getting popularity. Why don't you go to a lab where they are in >> use and take a look? >> RenéJ. >> >> From:"Huggins, Haley - MRMC" <[email protected]> >> To: "[email protected]" < >> [email protected]> >> Sent: Wednesday, September 11, 2013 4:46 PM >> Subject: [Histonet] Equipment selection >> >> Hi all, >> >> I am trying to decide on what equipment to purchase. I have picked >> the processor, microtomes, embedding centers and a few other pieces, >> but now I still need to decide on the H&E Stainer and the >> immunostainer. My options I have in front of me are for the H&E >> stainer are Sakura Prisma stainer/film coverslipper and the Leica >> ST5020-CV5030 stainer/glass coverslipper. For the Immunostainer, I am >> looking at the Dako Autostainer and the Leica Bond Max. Any advice on >> any of these instruments would be greatly appreciated. I am trying to >> finalize my decision pretty quickly. Thank you in advance for any input. >> >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> --------------------------------------------------------------------- >> - Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended recipient(s) and may >> contain confidential and privileged information. Any unauthorized >> review, use, disclosure or distribution is prohibited. If you are not >> the intended recipient, please contact the sender by reply e-mail and >> destroy all copies of the original message. >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Thu, 12 Sep 2013 11:45:41 -0500 >> From: "Webb, Dorothy L" <[email protected]> >> Subject: [Histonet] Need your expertise >> To: "'[email protected]'" >> <[email protected]> >> Message-ID: >> < >> 65365f35c0f2ef4d846ec3ca73e49c4302aceff2c...@hpemx3.healthpartners.in >> t> >> >> Content-Type: text/plain; charset="us-ascii" >> >> We will finally be going green by recycling formalin. I would >> appreciate any advice or opinions on true recycling VS. a filtering >> type of system. As we all know, experience with a system or product >> is the best way to gain true knowledge! >> >> Also, I would appreciate knowing how others are handling extra levels >> of prostate needle bx's. We currently place them on charged slides >> and keep until the case is signed out, but it seems there should be a >> better way of handling this due to storage of the slides not to >> mention the extra cost involved! I know this is becoming more of a >> needed process with all of the RNA/DNA testing being done on diagnosed >> cancers. >> >> As always, I do appreciate any and all comments, advice, or words of >> wisdom!! Hope to see some of you in Providence late next week! >> >> Dorothy Webb, HT (ASCP) >> Regions Histology Technical Specialist >> 651-254-2962 >> >> >> >> ________________________________ >> This e-mail and any files transmitted with it are confidential and >> are intended solely for the use of the individual or entity to whom >> they are addressed. If you are not the intended recipient or the >> individual responsible for delivering the e-mail to the intended >> recipient, please be advised that you have received this e-mail in >> error and that any use, dissemination, forwarding, printing, or >> copying of this e-mail is strictly prohibited. >> >> If you have received this communication in error, please return it to >> the sender immediately and delete the original message and any copy >> of it from your computer system. If you have any questions concerning >> this message, please contact the sender. Disclaimer R001.0 >> >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 12 Sep 2013 11:55:43 -0500 >> From: Tim Higgins <[email protected]> >> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23 >> To: "[email protected]" >> <[email protected]> >> Message-ID: <[email protected]> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hey Martha, >> >> You have to forgive Rene J. she speaks sometimes and has nothing good >> to say but keeps speaking. There is never a bad question, if you >> don't have a good answer then keep it to yourself as I usually do. >> >> "Adequate forum". really? I guess no one on Histonet is allowed to >> share their personal protocols, procedures, opinions, and experiences >> on here anymore. >> >> Come on Rene J., next time shhhhhhhhh!! >> >> I should have just hit the "Delete" button and follow my own advise! >> >> Tim N. Higgins, HT (ASCP), QIHC >> >> >> > Message: 7 >> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT) >> > From: Rene J Buesa <[email protected]> >> > Subject: Re: [Histonet] Minimum sample size for breast markers >> > To: Martha Ward-Pathology <[email protected]>, >> > "[email protected]" >> > <[email protected]> >> > Message-ID: >> > <[email protected]> >> > Content-Type: text/plain; charset=iso-8859-1 >> > >> > I do not think this is the adequate forum to ask this question >> > because >> you may get a wrong answer that could determine your decision. >> > Your pathologist is the one who should decide about this issue. >> > René J. >> > >> > >> > ________________________________ >> > From: Martha Ward-Pathology <[email protected]> >> > To: "[email protected]" < >> [email protected]> >> > Sent: Thursday, September 12, 2013 9:06 AM >> > Subject: [Histonet] Minimum sample size for breast markers >> > >> > >> > >> > >> > Is there a minimum number of cells that should be present when >> > testing >> for ER, PR and Her2? I have looked on the CAP website but cannot see >> anything about this. We are constantly getting these small biopsies >> or FNAs for testing and I sometimes wonder what the lowest threshold >> is for >> testing...20 tumor cells, 50 tumor cells???? Any information would >> be appreciated. Thanks in advance for your help. >> > >> > Martha Ward, MT (ASCP) QIHC >> > Manager >> > >> > Molecular Diagnostics Lab >> > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 >> > \ f 336.716.5890 [email protected] >> > >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 118, Issue 24 >> ***************************************** >> > > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
