I'd go with "A", but it really depends on what you are going to do with the sections after fixation.
In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular H&E and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 [email protected]<mailto:[email protected]> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
