On my IHC validation documents I have a space for comments.  If I change 
anything in a protocol I make a note in the comments space.  I haven't had any 
issue with any accrediting agencies.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
[email protected]
732-839-3374
Cell: 610-809-6508


________________________________________
From: [email protected] 
<[email protected]> on behalf of Laurie Colbert 
<[email protected]>
Sent: Tuesday, March 11, 2014 7:27 PM
To: Histonet Post ([email protected])
Subject: [Histonet] IHC Validation

If I have validated an antibody at a specific incubation time, and then later 
want to decrease the incubation time, do I have to re-validate??

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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