Long time and high volume user of the Sakura Tape. Check the Xylene drops. We coverslip thousands of slides daily and have seen no isssue with the tape lots. Our experience shows some cornflaking when the slide does not receive enough xylens to wet the slide properly.
William DeSalvo, BS HTL(ASCP) > From: [email protected] > To: [email protected]; [email protected]; > [email protected]; [email protected] > Date: Thu, 13 Mar 2014 16:13:24 +0000 > CC: > Subject: [Histonet] RE: Cornflaking artifact > > Can anyone share the lot # on their coverslipping film (Sakura)? We are > seeing this as well out of the blue..haven't changed a thing. > > Kenneth G Metzger HTL(ASCP) > Histology Supervisor > ARUP Labs > Salt Lake City, Utah > Phone: (801)583-2787 ext. 3101 > Fax: (801) 584-5244 > Email: [email protected] > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of HERRINGTON, > SHEILA > Sent: Thursday, March 13, 2014 9:42 AM > To: 'Laurie Colbert'; Sharon Scalise; [email protected] > Subject: [Histonet] RE: Cornflaking artifact > > We also have recently started to see this artifact more than ever before, and > nothing in our process has changed. We have tried everything to correct to > no avail. Wonder if it is possible to be a change in some type of supply, > either xylene or coverslipping film. Something has changed but am at a loss > as to what. > > > Sheila Herrington > Technical Lead Histopathology and Immunohistochemistry Kelowna General > Hospital > 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 > 250-862-4300 ext 7587 or 7510 > [email protected] > > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Laurie Colbert > Sent: Thursday, March 13, 2014 6:30 AM > To: Sharon Scalise; [email protected] > Subject: [Histonet] RE: Cornflaking artifact > > You will also see the cornflaking if your tissue is lifting off of the slide > at all. We used to get this more often on hard, decal specimens than on > other specimens. We used the film to coverslip. If you remove the film from > the problem slides and recoverslip conventionally with extra mountant and > glass coverslips, I'm sure you will not see the artifact. > > Laurie Colbert > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Sharon Scalise > Sent: Wednesday, March 12, 2014 8:00 PM > To: [email protected] > Subject: [Histonet] RE: Cornflaking artifact > > I am looking for help with "cornflaking" (tiny, brown dry spots under > coverslip)artifact. We have been using fresh xylene on our stainer and > coverslipper, cleaned and wiped all containers dry before filling, tried > different lots of coverslipping film and had service on our coverslipper to > make sure it was functioning properly, including the xylene drip. We > continue to have this artifact and it is driving us crazy. It is sporadic > with no pattern of tissue type or placement on the slide. Sometimes it lands > on tissue other times not. Most of the time when we remove the coverslip and > re-coverslip it goes away (I am assuming because the acetone removes any > minute amounts of water that may be present). We just cannot figure out > where the water is coming from. Has anyone seen this artifact while using > the drying step on the prisma stainer? We just recently started using the > drying on some slides and I am thinking maybe it is causing humidity??? I > cannot say for a fact that our "cornflaking" started at the same time, but it > is suspicious. HELP!!!!! > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Wait, Trevor > Jordan > Sent: Wednesday, March 12, 2014 3:57 PM > To: [email protected] > Subject: [Histonet] Paraffin type and Tetracycline labelling Questions > > For those who have done Decalcified bone processing with paraffin....what is > the best type of paraffin that you guys are familiar with? > > Also, if you are wanting to see a tetracycline label on the bone for bone > turnover, must undecalcified sections be used? How for a double tetracycline > label? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD > Candidate Abilene Christian University Class of 2013 Graduate B.S. > Biochemistry _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------- > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
