Hello all, Please I need some help in the lipophilic dil labeling of free floating brain sections. The mice brain were fixed in 1.5% PFA by cardia perfusion, then left one hour in the same fixative. I used the Dil labeling solution for a 200 um thick sections, the dilution was 1:150 in PBS. The sections were incubated for 48h in the Dil labeling solution then 48 in PBS in the cold room in the shaker. The problem that I had a very high background and I could not see even one spine. I need to stain only a few neurons so I can do a reasonable quantification of the size and number of the spines. Does anybody has an idea if in this case the solution or the crystal Dil is better and can I get an optimized protocol for this kind of staining. Thank you so much All the best Sent from my iPad _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
