My story - I finished my degree late in my career and just wanted to take the registry to see how it was - then I was going to know how to study. I didn't spend much time studying - just brushing up as I took it soon after I graduated and had been doing thesis type reports, etc. I got to the exam - I had to go 100 mi to Knoxville from Kingsport, TN - and they had scheduled me for the wrong day and had all the nursing students there taking their Boards. They told me I could stay and wait till a computer was available - I did. I had taken those books with me, and reviewed as I sat there - learned all kinds of things I used on the test! I passed and didn't have to take it again. It was a miracle!! j
Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax [email protected] www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Campbell, Tasha M. [mailto:[email protected]] Sent: Wednesday, May 21, 2014 9:48 AM To: Weems, Joyce K.; [email protected]; [email protected] Subject: RE: [Histonet] HTL Definitely the booklets from NSH. Probably the most important, I think. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 21, 2014 8:26 AM To: '[email protected]'; [email protected] Subject: RE: [Histonet] HTL Also the booklets from NSH .. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax [email protected] www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, May 21, 2014 6:54 AM To: [email protected] Subject: [Histonet] HTL &nb=;WHen I took mine i did both parts. for the written carson cover to cover=d the thick bancroft and stevens(sp?) 2 times cover to cover. Nick(Rocky) Madar= HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) <=v style="border-top:1px solid #bcbcbc;margin:5px 0px;"> On 05/20/14, [email protected] wrote: Send Histonet =iling list submissions to [1][email protected]=estern.edu To subscribe or unsubscribe via the World Wide =b, visit [2]http://lists.utsouthwe=ern.edu/mailman/listinfo/histonet or, via email, send a message =th subject or body 'help' to [3][email protected] You can reach the person man=ing the list at [4][email protected]=hwestern.edu When replying, please edit your Subject line = it is more specific than "Re: Contents of Histonet digest...&q=t; Today's Topics: 1. HTL certification (C y Stan) 2. RE: HTL certification (Esther C Peters) 3. pos=ive control indoleamine 2-3 dioxygenase (Pathology-Histology S= Supervisor) 4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials (Clare Thornton) 6. RE: antibody vials (Mu=hy, Valerie) 7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya) 8. new processor (anita) 9. RE: antibody vials (A=e Murvosh) 10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya) 11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya) ----------------------------------------------- ----------------------- Message: 1 Date: Mon, 19 May 2014 1=32:36 -0500 From: Cecy Stan <[5][email protected]> Subject: [Histonet] HTL certification To: [6]histone=lists.utsouthwestern.edu Message-ID: <CACh=0wgaaEfV DFmsuB-Jw8OKo=LKUP[7][email protected]= Content-Type: text/plain; charset=UTF-8 Hello ev=yone, I'm starting to prepare for my HTL certification (I am v=y nervous and anxious but also very excited about this decision to g=or it). I was just curious to know how you guys prepared for =, and how long it took for you to prepare before taking the test. Wi= 6 months preparation be enough? (I know that may depend on the indi=dual; it's just that I had my Masters over 10 years ago and I haven'=tudied this much since then). I have Freida L. Carson's =d Edition book -- quite daunting to memorize -- but the outline ASCP=s provided for study seems to be helpful. Do you have any other bo=/study aid suggestions? Thank you in advance for your input an dvice! C.A. ------------------------------ Message: 2 Date: Mon, 19 May 2014 17:46:47 +0000 From: Esther=eters <[8][email protected]> Subject: RE: [Histonet] HTL ce=ification To: Cecy Stan <[9][email protected]>, =9"[10][email protected]" <[11][email protected]> Me=age-ID: <[12][email protected]> Content-=pe: text/plain; charset="iso-8859-1" Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memoriz=g is not what you should be doing. You need to understand concepts, so th= when you need to troubleshoot problems you will be able to think through=ings, rule some things out, and make sense of the situation. I see this =l the time with my students, they forget things they memorize, but then t=y finally understand things and can figure things out. One of the new tea=ing tools is having students prepare "concept maps," to see the=lationships of topics and terms, and these linkages will help you in the=ng run. For histology examples, see: [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html I don't know of any concept maps for his=technology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Profes=r Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: D=id King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066e-mail: [14][email protected] [15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu ______________=________________________ From: [16][email protected] <[17]histonet-b [email protected]> on behalf of Cecy Stan <[18]cec [email protected]> Sent: Monday, May 19, 2014 1:32 PM To: =9][email protected] Subject: [Hist=et] HTL certification Hello everyone, I'm starting = prepare for my HTL certification (I am very nervous and anxious but=so very excited about this decision to go for it). I was just=rious to know how you guys prepared for it, and how long it took fo=ou to prepare before taking the test. Will 6 months preparation be =ough? (I know that may depend on the individual; it's just that I ha=y Masters over 10 years ago and I haven't studied this much since t=n). I have Freida L. Carson's 3rd Edition book -- quite daunti= to memorize -- but the outline ASCP has provided for study seems t=e helpful. Do you have any other book/study aid suggestions? <= />Thank you in advance for your input and advice! C.A. _______=______________________________________ Histonet mailing list [20][email protected] [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Messa=: 3 Date: Tue, 20 May 2014 12:09:28 +0000 From: "Pathology=istology Sr. Supervisor" <[22][email protected]> Subject:=istonet] positive control indoleamine 2-3 dioxygenase To: "[23][email protected]" <[24][email protected]> Cc: "[25][email protected]" <[26][email protected]>Message-ID: <BBFD4ECF5CBFAE4E936663D [email protected]> Content-Type: text/plain;=arset="us-ascii" Hi All, We are going to opt=ize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue= immunohistochemistry on formalin fixed paraffin sections. I shall thankful if any one please let me know about the "positive control= Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCH&RC Lahore Pakistan -----------------------=----- Message: 4 Date: Tue, 20 May 2014 13:03:55 +0000 From: Michael LaFriniere <[27][email protected]= Subject: [Histonet] immunohistochemisty coding To: "=[28][email protected]'" <[29]Sarah.Dysart @stdavids.com>, "[30][email protected]=western.edu" <[31][email protected]=estern.edu> Message-ID: <4A2A16B9707CE04E9C[32][email protected] cal> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Joyce is correct, under medicare changes in patho=gy coding, you can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the patholo= report is allowable. Medicare immuno billing is per "specimen"=ly for each specific antibody used, example if you used s100 for the sam=pecimen (wide melanoma excision)on 15 different blocks, under medicare y= can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen = the immunohistochemistry world and the labs that have capitalized on the=actice, the problem with the lower reimbursements and change in medicare=ding hurts many of our labs who have used the utilization of immunohisto=emistry appropriately. However, "under current 2014&=ot; CPT coding on non medicare can you bill for the additional work per b=ck using the 88342 and 88343 cpt code, I am sure moving forward we are go=g to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suit * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844 [33][email protected] -----Original Message----- From: [34][email protected] [ma ilto:[email protected]] On Behalf Of Weems, Joy K. Sent: Monday, May 19, 2014 12:21 PM To: '[35][email protected]'; [36][email protected] Subject: [Histonet] RE: Billing No - one G0461. <= />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462=. j Joyce Weems Pathology Manager 678-843-7376 Pho= 678-843-7831 Fax [37][email protected]= [38]www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-ma=, including any attachments is the property of Saint Joseph's Hospital an=s intended for the sole use of the intended recipient(s). It may contai=nformation that is privileged and confidential. Any unauthorized review=se, disclosure, or distribution is prohibited. If you are not the intend recipient, please delete this message, and reply to the sender regarding=e error in a separate email. -----Original Message----- =om: [39][email protected] [[40]mailto:[email protected]=] On Behalf Of [41][email protected] Sent: Mon y, May 19, 2014 12:00 PM To: [42][email protected]=estern.edu Subject: [Histonet] Billing When it comes = the medicare codes...question... So you have one specimen tha=as 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bil=E1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine t=es? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St. David's North Austin Medical Center 1222=orth Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing=st [43][email protected] [44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet ________________________________ Th= e-mail message (including any attachments) is for the sole use of t= intended recipient(s) and may contain confidential and privileged i=ormation. If the reader of this message is not the intended recipien= you are hereby notified that any dissemination, distribution or cop=ng of this message (including any attachments) is strictly prohibite= If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the or=inal message (including attachments). ________________________=_____________________ Histonet mailing list [45][email protected] [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet <= /> ------------------------------ Message: 5 Date: =e, 20 May 2014 10:48:20 -0400 From: Clare Thornton <[47][email protected]> Subject: [Histonet] antibody vials To:=48][email protected]" =9<[49][email protected]> Mes=ge-ID: <C9D78FFC9D668B4CBEA4405F[50]84697 [email protected]> Content-Type: text/plain; c=rset="us-ascii" Does anyone have any good ideas fo=eeping the little vials of concentrated antibodies neat and organized in=e fridge? Right now we keep ours in empty pipette tip boxes (without ti=olders) but they're always falling over and mixed up. We use several di erent vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas! Clare J. Thornton=TL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase=agnostic Services 417 State Street, Suite 540 Bangor, ME 04401 [51][email protected]<[52]mailto:cthornton@dahl=ase.com> ----------------------------= Message: 6 Date: Tue, 20 May 2014 14:59:21 +0000 Fr=: "Murphy, Valerie" <[53][email protected]> =bject: [Histonet] RE: antibody vials To: Clare Thornton <[54][email protected]>, "histonet@l=ts.utsouthwestern.edu" <[55]histonet@li=s.utsouthwestern.edu> Message-ID: <A0CD799E6FCD494 AD4[56][email protected] armanos.org> Content-Type: text/plain; charset="us-ascii= I use cardboard cryovial boxes and they work quite well. =u can remove some of the dividers to make space for the larger vials. [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre eze=Racks/Cryovial-Boxes-Cardboard/ Valerie =tliff B.Sc HTL(ASCP) Research Assistant Department= Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: [58][email protected]= Better treatments. Better outcomes. ________________________________________ From: [59][email protected] [[60][email protected]] on behalf of Clare Thornto=[61][email protected]] Sent: Tuesday, May 20, 2014 1=48 AM To: [62][email protected] Subject: [Histonet] antibody vials Does anyone have any good =eas for keeping the little vials of concentrated antibodies neat and orga=zed in the fridge? Right now we keep ours in empty pipette tip boxes (wi=out tip holders) but they're always falling over and mixed up. We use se=ral different vendors for our concentrated antibodies, so our vials are a= different sizes. Thanks for any ideas! Clare J. =ornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Da=-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, = 04401 [63][email protected]<[64]mailto:cthornto=dahlchase.com> __________________________________=___________ Histonet mailing list [65]Histonet@li=s.utsouthwestern.edu [66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet ----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain confiden=al and/or privileged information. If you are not the intended recipient(s= you are hereby notified that any dissemination, unauthorized review, use=isclosure or distribution of this email and any materials contained in a= attachments is prohibited. If you receive this message in error, or are =t the intended recipient(s), please immediately notify the sender by emai= and destroy all copies of the original message, including attachments. ------------------------------ Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT) From: "Balasubbra=nian, Dakshnapriya" <[67][email protected]><= />Subject: [Histonet] HELP- Cryosectioning FAT! To: [68][email protected] Message-ID: <21128R24.17352091.1400599[69][email protected]> Content-Type: text/plain; charset=utf-8 Hi all, I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is probab= a much discussed topic, but I've tried a lot of strategies with no luck.=e tissue is of mouse origin and has micro-lesions buried inside fat. So =e fat needs to be cut in order to get to the lesion. I started with a tem=rature of -17C and tried upto -25C and also at -50C; didn't work at any o=hese temperatures. I tried rubbing the block, blade and anti-roll plate =th dry ice-again,no luck. I rapid-freeze the tissue with OCT = LN2 and then store the block at -80C. Could I try sectioning the block d=ectly from -80C? (never done that). Any other suggestions on =w to tackle this? Any advice is much appreciated!! =anks, Dakshna ------------------------------<= /> Message: 8 Date: Tue, 20 May 2014 10:26:13 -0500 From:=ita <[70][email protected]> Subject: [Histonet] new p=cessor To: "[71][email protected]= <[72][email protected]<=>> Message-ID: <COL131-W4520B92[73]279A33E8D42CCF0D[email protected]> Content-Type: text/plain; charset="iso-885=1" Thanks everyone for your input on processors, I think= will stay with the VIP, it has been a good machine just getting older n=. everyone have a great day!!!! Anita Dudley Providence Hosp Mobile, Alabama 3f95 ------------------------------<= /> Message: 9 Date: Tue, 20 May 2014 08:29:05 -0700 From:=nne Murvosh" <[74][email protected]> Subject: RE: [Histonet] antibody vials To: "Clare Thornton"=[75][email protected]>, <[76][email protected]> Message-ID: <4A A4E531E8C943A7[77][email protected]=et> Content-Type: text/plain; charset="us-ascii"= We got some plexiglass dividers at the "Container Store&q=t; if you have one near or check online. Can't remember what they w=e called but they had different sized square compartments that fit v=ious venders antibodies. Anne -----Original Message-----<= />From: [78][email protected]. u [[79]mailto:[email protected]=estern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May , 2014 7:48 AM To: [80][email protected]= Subject: [Histonet] antibody vials Does anyone have =y good ideas for keeping the little vials of concentrated antibodies=at and organized in the fridge? Right now we keep ours in empty pi=tte tip boxes (without tip holders) but they're always falling over =d mixed up. We use several different vendors for our concentrated a=ibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohi=ochemistry Technologist Dahl-Chase Diagnostic Services 417 Stat=treet, Suite 540 Bangor, ME 04401 cthornton@dahlchase.=m<[81]mailto:[email protected]> _=____________________________________________ Histonet mailing list[82][email protected] [83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet ------------------------------ Message: 10 Date: Tue, 20 May 2014 10:59:16 -0500 (CDT) From: =alasubbramanian, Dakshnapriya" <[email protected]=u.edu> Subject: Re: [Histonet] HELP- Cryosectioning FAT! To: "Sandra E. Esparza" <[84][email protected]>, =9[85][email protected] Message-ID: <601376475.17568918.1400601[86][email protected]> Content-Type: text/plain; charset=utf-8 Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza"=[87][email protected]> To: "Dakshnapriya Balasubbram=ian" <[88][email protected]> Sent: Tues y, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectio=ng FAT! You might want to dry Liquid Nitrogen on the face of t= block before you cut it. Sandra Sandra Esparza H=ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery nter 512-324-7468 x84027 [89][email protected] -----Original Message----- From: [90][email protected] [[91]mailto :[email protected]] On Behalf Of Balasubbramani=, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: [92][email protected] Subject: [Histonet] =LP- Cryosectioning FAT! Hi all, I've been having pr=lems with cryosectioning fatty tissue for a long time now. The section le=es a hole in the middle. I know this is probably a much discussed topic, =t I've tried a lot of strategies with no luck. The tissue is of mouse ori=n and has micro-lesions buried inside fat. So the fat needs to be cut in =der to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tri rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b=ck at -80C. Could I try sectioning the block directly from -80C? (never d=e that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna <= />_______________________________________________ Histonet mailing =st [93][email protected] [94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet CONFIDENTIALITY NOTICE: This email m=sage and any accompanying data or files is confidential and may contain p=vileged information intended only for the named recipient(s). If you are =t the intended recipient(s), you are hereby notified that the disseminati=, distribution, and or copying of this message is strictly prohibited. If=u receive this message in error, or are not the named recipient(s), plea= notify the sender at the email address above, delete this email from you=omputer, and destroy any copies in any form immediately. Receipt by anyo= other than the named recipient(s) is not a waiver of any attorney-client=ork product, or other applicable privilege. -----=----------------------- Message: 11 Date: Tue, 20 May 201A1:03:08 -0500 (CDT) From: "Balasubbramanian, Dakshnapriya"= <[95][email protected]> Subject: Re: [Histon=] HELP- Cryosectioning FAT! To: Laurie J King <[96][email protected]>, histonet=ists.utsouthwestern.edu Message-ID: <262036458.175957e.1400601[97][email protected]> =ntent-Type: text/plain; charset=utf-8 Hi Laurie, =, the tissue hasn't been fixed before freezing. My prof doesn't prefer th=ethod, but if nothing else works, we might give it a try. An=uggested protocols for fixation? Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" << lass="parsedEmail" href="mailto:[email protected]"; ta rget="_blank">[email protected]> To: "Dak=napriya Balasubbramanian" <[98][email protected]> Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histo=t] HELP- Cryosectioning FAT! Dakshna, Has this tiss= been fixed before freezing by any chance? laurie -=--Original Message----- From: [99][email protected] [[100]mailto:[email protected]] On Behalf Of Balasubbramanian, Daksh=priya Sent: Tuesday, May 20, 2014 10:23 AM To: [101][email protected] Subject: [Histonet] HELP- Cryo=ctioning FAT! Hi all, I've been having problems wit=ryosectioning fatty tissue for a long time now. The section leaves a hol=n the middle. I know this is probably a much discussed topic, but I've t=ed a lot of strategies with no luck. The tissue is of mouse origin and ha= micro-lesions buried inside fat. So the fat needs to be cut in order to g= to the lesion. I started with a temperature of -17C and tried upto -25C =d also at -50C; didn't work at any of these temperatures. I tried rubbing=e block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -8 . Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any ad=ce is much appreciated!! Thanks, Dakshna _____=________________________________________ Histonet mailing list =02][email protected] [103]http://lists.utsouthwestern.edu/mailman/listinfo/histon= _________________________________________________________ _____________ The contents of this message may contain private, protec=d and/or privileged information. If you received this message in error, =u should destroy the e-mail message and any attachments or copies, and yo=re prohibited from retaining, distributing, disclosing or using any info=ation contained within. Please contact the sender and advise of the erro=ous delivery by return e-mail or telephone. 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