I'd advise checking your dehydration alcohols with a hydrometer. This "bleeding" happened to me once and it turned out the alcohols after the Eosin were not graded. My lab aide accidently used 100% only instead of 70%, 95% and then 100%. Anita
Anita -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Adrienne Anderson Sent: Friday, June 20, 2014 8:15 AM To: Rathborne, Toni Cc: [email protected] Subject: Re: [Histonet] Eosin Leaching We haven't changed our mounting medium, but I'll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it's very possible I didn't make that a point. I'll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni <[email protected]> wrote: > Is your alcohol level as high as or higher than the level of eosin in the > containers? If there is residual eosin left at the upper portion of the > slide, that could be causing the problem. > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Sanders, > Jeanine (CDC/OID/NCEZID) > Sent: Thursday, June 19, 2014 5:26 PM > To: 'Adrienne Anderson'; '[email protected]' > Subject: RE: [Histonet] Eosin Leaching > > Have you changed your coverslipping mountant? > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Adrienne > Anderson > Sent: Thursday, June 19, 2014 5:24 PM > To: [email protected] > Subject: [Histonet] Eosin Leaching > > Hello all, > > We're having a problem with our eosin bleeding out of the sections. I've read > that this can be caused by not dehydrating adequately after eosin, but we're > still having an issue when using fresh alcohols. Does anyone have any other > ideas as to the cause of this problem? > > Thanks in advance for your help! > > Adrienne > > > > Adrienne Anderson, BS, HTL(ASCP) > Histotechnologist > Phylogeny, Inc. > 1476 Manning Pkwy, Powell, Ohio 43605 > Phone: (614) 846-6161 > Fax: (877) 591-1815 > > This message, including any attachments, is confidential and may be > privileged or may contain health information protected by state and federal > law. > Information and opinions expressed in this message and/or attachments are > those of the author and are not necessarily those of the company. > If you are not the intended recipient, please notify the sender and delete > this message from your system. > Any use of this information by individuals other than the intended recipient > is strictly prohibited. _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
