As a first information, we use sliding microtomes with the knife set in an angel (called declination). We embed straight, because areas of same concistancy should be positioned in one line and in the cutting direction. Areas of more rigidity compress the smoother ones, if they are shifted in an angle.
If you have problems with the orientation of the tissue in the block, just turn the blockholder. Gudrun -----Ursprüngliche Nachricht----- Von: [email protected] [mailto:[email protected]] Im Auftrag von Sanjeet Gesendet: Donnerstag, 31. Juli 2014 03:46 An: [email protected] Betreff: [Histonet] Fwd: Embedding skin Sent from my iPhone Begin forwarded message: > From: Sanjeet <[email protected]> > Date: July 30, 2014 at 9:27:46 PM EDT > To: "[email protected]" <[email protected]> > Subject: Embedding skin > > Hi Histo techs > > Need some info on the correct orientation of skin tissue. Punch/ ellipse. Does anyone have a literature on this topic. I am used to embed large skin in an angle, the skin being on top. > Currently the place where I work have different protocol, the skin is embedded straight, the epidermis being right angled to the mold. > I find the section difficult to cut when the skin is embedded straight , the section are compressed and more chances scoring along the section. > > Thanks > Sanjeet > > Sent from my iPhone _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
