I've observed images on a computer screen with a person next to me insisting that the adipose tissue in H&E stained tissue has a color versus areas with no tissue (open areas). Am I missing something, or do others see either a slight discoloration in adipose tissue, or a slightly different color, versus areas without tissue? I suspect it depends on section thickness, but given a 5 - 8 micron section it seems that whatever tissue might have been present would be scraped away with the blade.

Jerry Sedgewick
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