Hi Donna, Interesting conundrum! A few questions: - Do you see it in tissues that have not undergone any staining, just floated in buffer and coverslipped? - Have you tried wet mounting them after staining and prior to dehydration and clearing to see if the artifact is there? - Have you recently changed glove manufacturers, could this be talc or some other powder from gloves? - Are you dehydrating out of PBS into an alcohol concentration higher than 70%? You could be getting phosphate precipitation. Try using a water rinse prior to alcohol or make sure your first alcohol concentration is 70% or lower.
Those are the weird things that came to mind. I hope this helps, Teri Johnson Manager Clinical Trial Testing Genoptix, Inc. Carlsbad, CA 92008 ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
