Increase the time and/or temperature. I bake mine at 85 Celsius for 20 minutes on non charged slides. Section thickness is also a factor as well as the pH of your blueing reagent.
-- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
