Oh, sorry, I forgot one additional question regarding Carnoy's: in the
protocols I found, Carnoy's is prepared using DRY methanol. Sorry for my
ignorance, but does this mean that methanol has to be specifically
treated to remove any hygroscopic etc water? Note that this is
specifically intended for preserving intestinal mucus, which I guess
might be sensitive to water-containing fixatives (although I presume
there can't be more water in ordinary methanol than introduced by the
tissue sample itself).
With best regards,
Mikael
On 21.11.2014 12:23, Mikael Niku wrote:
Hello!
What is the best way to fix intestinal samples in order to preserve
the mucus (and the embedded bacteria)?
I was recommended Carnoy's, but are there any alternatives... perhaps
without chloroform?
With best regards,
Mikael
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