Why don´t you use the classic Highman´s Congo red hidroalcoholic solution? (0,5% in 80% ethanol). Best luck, Carlos.
*********** REPLY SEPARATOR *********** On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote: >Hi All: > >Lately, I have been having problems with my Congo Red staining too lightly. >The Bielschowsky silver stain shows lots of amyloid in the cerebral blood >vessels and the cores of the senile plaques. >However, the Congo Red stain is pretty faint, although you can still see >it. >I have just bought a new bottle of Congo red from Sigma, in case it was >the particular batch of dye. >My protocol, which I have always used, is as follows: > >1. Deparaffinize sections and bring down to 95% ethanol >2. Stain in 1% Congo Red solution for 10 minutes. >3. Differentiate in Potassium Hydroxide solution; 3 dips >4. Rinse in 4-5 changes of tap water. >5. Counter-stain in Harris Hematoxylin for 1 minute. >6. Differentiate in Acid Alcohol; 10 dips or so. >7. Blue Hematoxylin in running tap water for 10 minutes. >8. 95% ethanol for only 3 dips, then right into absolute ethanol. >9. 20 dips in first absolute ethanol >10. 1 minute in absolute ethanol >11. Clear and mount. > >Any suggestions would be really appreciated, > >Tim Wheelock >Harvard Brain Bank >Mclean Hospital >Belmont, MA > > >The information in this e-mail is intended only for the person to whom it >is >addressed. If you believe this e-mail was sent to you in error and the >e-mail >contains patient information, please contact the Partners Compliance >HelpLine at >http://www.partners.org/complianceline . If the e-mail was sent to you in >error >but does not contain patient information, please contact the sender and >properly >dispose of the e-mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet