Akemi, it is really up to the pathologists to decide what they want. In some 
cases there is literature promoting certain standards (e.g, Hirschprung's) but 
in most cases it is just the comfort level of the person reviewing the case. We 
have a large variety of levels and unstained protocols but for standard 
biopsies it is two levels on one slide, no unstained. We only cut unstained if 
they are ordered as part of a protocol or ordered specifically. We do not cut 
"extra" unstained "just in case." We had tens of thousands of unstained slides 
sitting in files "just in case" so I convinced them to stop that practice. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi 
Allison
Sent: Friday, January 09, 2015 6:07 AM
To: Histonet
Subject: [Histonet] Levels for Gastic and Esophogeal Bx's

Good morning Histoland:

Happy Friday!  I believe this question was asked last year, but I would like to 
revisit this again. I have worked in a variety of laboratories in my career, 
both small and large. Most of my experience was in Oregon with large hospital 
laboratories and University institutions. Back then, we cut (6) levels on all 
our bx’s, and picked-up (2) sections on each level.  My current facility is 
cutting (3) levels and picking up (1) section on each level.

I would like to ask what everyone else is doing.  The reason I am asking is 
because our pathology director is requesting a change in our protocol to cut 
only (2) levels and (1) section at each level.

Sincerely,

Akemi Allison BS, HT/HTL (ASCP)

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