Eosin can be a problem in IHC as well especially if you use alk phos red detection.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 [email protected] > From: [email protected] > To: [email protected] > Date: Wed, 14 Jan 2015 22:56:51 +0000 > Subject: Re: [Histonet] marking small samples > CC: [email protected]; [email protected] > > I've been told Eosin affects a lot of molecular testing. We used to use it in > our process but discontinued to not interfere with future testing. > > Sent from my iPhone > > > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" <[email protected]> > > wrote: > > > > We use eosin the processor alcohol. Also we use blue-tinted paraffin which > > helps with contrast on small bx > > > > Tim Morken > > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > > UC San Francisco Medical Center > > San Francisco, CA > > > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is > > for the sole use of the intended recipient(s) and may contain confidential, > > proprietary, and/or privileged information protected by law. If you are not > > the intended recipient, you may not use, copy, or distribute this email > > message or its attachments. If you believe you have received this email > > message in error, please contact the sender by reply email and destroy all > > copies of the original message. > > > > > > -----Original Message----- > > From: [email protected] > > [mailto:[email protected]] On Behalf Of Yves > > Heremans > > Sent: Wednesday, January 14, 2015 2:03 PM > > To: [email protected] > > Subject: [Histonet] marking small samples > > > > Dear Histonetters, > > > > Does anyone know of a good method to mark (stain) small samples (tissue or > > cells) prior to paraffin embedding to aid in finding back more easily the > > sample in the paraffin block ? > > Preferably something that would not interfere with subsequent antibody > > staining. > > > > Yves > > > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
