Hi Jeffery: Yes, I notice the same "paraffin dust bunnies", perhaps especially since I use a embedding paraffin that has a fair amount of plastic. Before I embed my brain tissue, I mix the embedding media thoroughly, until the solution is clear.
As for your Congo Red problem, I wish that I could help you. I am now experiencing the same problem, except that it affects the positive controls as well. The staining is there, but much fainter than it should be. I usually immerse albumin coated slides in 1% Congo red for 15 minutes, differentiate them with Potassium Hydroxide for 3 dips, counterstain with Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol for 2 dips, then dehydrate and mount. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
