Re: Frozen Sections The histo techs at our facility help our pathologist(s) as well as our Pathologist Assistant with the cutting and staining of frozen sections.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, February 08, 2015 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. TRAP staining for ffpe sections (abt...@gmail.com) 2. decalcifying sheep knee joints (abt...@gmail.com) 3. Cryosectioning undecal none (abt...@gmail.com) 4. Why when I submit my answer to the list an email with Korea language pop up? (abt...@gmail.com) 5. Re: Frozen Sections (histot...@imagesbyhopper.com) 6. RE: it happened again - the foreign reply (Joelle Weaver) 7. RE: CLIA Inspection Question (Joelle Weaver) 8. Re: CLIA Inspection Question (Garreyf) 9. Re: Frozen Sections (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Feb 2015 14:16:58 -0500 From: abt...@gmail.com Subject: [Histonet] TRAP staining for ffpe sections To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <a833c77b-c089-4c9d-bfbd-71b5c4807...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, I make staining solution for TRAP staining. Used once Sigma kit, but unsatisfactory. You may Google for TRAP protocol online. It must be there and easier than the kit. Dorothy Hu Manager of histology and histomorphometry operation HSDM Sent from my iPad ------------------------------ Message: 2 Date: Sat, 7 Feb 2015 14:26:28 -0500 From: abt...@gmail.com Subject: [Histonet] decalcifying sheep knee joints To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3c2ad062-414b-4bcf-a57c-bc33a2603...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, You may use formic acid instead of EDTA, it will be much fast and give you sharp image of Safranin O. You can also do IHC. Dorothy Hu HSDM Sent from my iPad ------------------------------ Message: 3 Date: Sat, 7 Feb 2015 14:34:13 -0500 From: abt...@gmail.com Subject: [Histonet] Cryosectioning undecal none To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f3844dc9-b925-424f-8fdc-ecba2ac81...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, We are using Kawamoto's Section-Lab Co. Ltd. products now. It is better than cryojane. Check Kawamoto's paper online to get protocol. Good luck. Dorothy Hu HSDM Sent from my iPad ------------------------------ Message: 4 Date: Sat, 7 Feb 2015 14:37:24 -0500 From: abt...@gmail.com Subject: [Histonet] Why when I submit my answer to the list an email with Korea language pop up? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bb85ad27-7e3b-41f3-bc25-ddc985aa3...@gmail.com> Content-Type: text/plain; charset=us-ascii Can it be blocked? Thanks. Dorothy Sent from my iPad ------------------------------ Message: 5 Date: Sat, 07 Feb 2015 17:01:26 -0500 From: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com> Subject: Re: [Histonet] Frozen Sections To: "lsmal...@juno.com" <lsmal...@juno.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <abc51eae-6ca7-4fbf-9f98-e0ca7c8ed...@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, "lsmal...@juno.com" <lsmal...@juno.com> wrote: Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sat, 7 Feb 2015 22:12:59 +0000 From: Joelle Weaver <joellewea...@hotmail.com> Subject: RE: [Histonet] it happened again - the foreign reply To: Michelle <histot...@imagesbyhopper.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <snt149-w481282aa57e51d67aed945d8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I get the same thing everytime. No, I don't know why it appears. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: histot...@imagesbyhopper.com > To: Histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 10:01:24 -0500 > CC: > Subject: [Histonet] it happened again - the foreign reply > > Does anyone have a clue as to why I keep getting a "foreign" reply to > every email I send to histonet? Is the group seeing the same foreign > message I am seeing? Am I the only one getting this message? > > > > Michelle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Sat, 7 Feb 2015 22:39:58 +0000 From: Joelle Weaver <joellewea...@hotmail.com> Subject: RE: [Histonet] CLIA Inspection Question To: Michelle <histot...@imagesbyhopper.com>, 'Bryan Szpunar' <bszpu...@umail.iu.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <snt149-w40310a48a5bd6cd9d4ee8dd8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Probably depends on your environment or organization, but personally I would go for CAP if you are doing pathology. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: histot...@imagesbyhopper.com > To: bszpu...@umail.iu.edu; histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 09:30:34 -0500 > Subject: RE: [Histonet] CLIA Inspection Question > CC: > > Hi Histonetters, > > It's me again! ;) I am interested in your thoughts on this: would it be > better to simply apply for a CAP accreditation and get both the CAP and CLIA > certificates at the same time? My thoughts are these: if meeting the CAP > guidelines is effectively meeting the CLIA requirements, would it make more > sense to prep for one bird and get two at the same time? Would I still need > to look at the CLIA regs, under this scenario? > > Out of my element, but definitely trying to learn! > > Thanks for the input I have received so far ... you all are a > wonderful resource. :) > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Sat, 7 Feb 2015 23:09:48 -0500 From: Garreyf <garr...@gmail.com> Subject: Re: [Histonet] CLIA Inspection Question To: Joelle Weaver <joellewea...@hotmail.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Bryan Szpunar <bszpu...@umail.iu.edu> Message-ID: <76be599b-f46f-4982-9de6-f675cff96...@gmail.com> Content-Type: text/plain; charset=us-ascii I believe you apply for a clia certificate regardless. Then, every two years you need to be inspected. You can choose cap or joint commission to survey you. Cap meets or exceeds clia regulations. I'm more familiar with CAP. If you choose cap your lab will be required to inspect another lab. Hope this helps. Garrey Sent from my iPhone > On Feb 7, 2015, at 5:39 PM, Joelle Weaver <joellewea...@hotmail.com> wrote: > > Probably depends on your environment or organization, but personally I would > go for CAP if you are doing pathology. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > >> From: histot...@imagesbyhopper.com >> To: bszpu...@umail.iu.edu; histonet@lists.utsouthwestern.edu >> Date: Sat, 7 Feb 2015 09:30:34 -0500 >> Subject: RE: [Histonet] CLIA Inspection Question >> CC: >> >> Hi Histonetters, >> >> It's me again! ;) I am interested in your thoughts on this: would it be >> better to simply apply for a CAP accreditation and get both the CAP and CLIA >> certificates at the same time? My thoughts are these: if meeting the CAP >> guidelines is effectively meeting the CLIA requirements, would it make more >> sense to prep for one bird and get two at the same time? Would I still need >> to look at the CLIA regs, under this scenario? >> >> Out of my element, but definitely trying to learn! >> >> Thanks for the input I have received so far ... you all are a >> wonderful resource. :) >> >> Michelle >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Sun, 8 Feb 2015 09:40:27 -0500 From: Bob Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Frozen Sections To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <CAOKsRH5Qz0GZgV2qQ9O=d0w4sdor8xk6nfyxtraq-zmmgoz...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Lorraine asks: >>Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist?<< This has to do more with the prestige of the pathologist(s) in the laboratory than with the actual need. Even if I'm doing the frozen section myself, I appreciate having a second person to stain the slide - it considerably improves turnaround if I'm having trouble cutting the section. Many younger pathologists do not have adequate skills in cutting frozen sections, and really need somebody to cut the sections. I cut hundreds of them during my residency, and thousands during my research fellowship, but not every pathologist has this experience. On a different topic, most formaldehyde neutralizers are simply sodium bisulfite (a.k.a. metabisulfite), but you have to have the cachet of an expensive brand-name to keep the inspectors and managers happy. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 9 **************************************** [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. § 75-24-29), the Privacy Act of 1974 (5 U.S.C. § 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. 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