The answer is either neutral EDTA for several weeks or in an acid for fewer weeks. It's all there in the books!
There's no quick way to decalcify a big bone. Nothing is "special" about safranine O. It is a red cationic dye that attaches to tissue anions such as nucleic acids (cell nuclei) and polyanions in mucus, bacterial and plant cell walls etc. John Kiernan London, Canada = = = On 06/02/15, Fiona J Wright <[email protected]> wrote: > Can anyone help me out with a problem Im going to have in a few months? I > will be receiving some sheep joints for processing. They will need to be > decalcified in a manner that will leave the tissue suitable for > immunohistochemistry and special stains such as safranin O. How do I go > about this? I have previously only worked with mouse bones and decalcified > for several weeks in EDTA; this has always given me good histological > results, but I fear for sheep bones this methodology would take months. > > Any advice gratefully received. > > Fiona Wright > > Dept of Infection and Immunity > University of Sheffield > K118, Medical School > Beech Hill Road > Sheffield > S10 2RX > [email protected] > 0114 271 2102 > 07769 334438 > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
