Debra, it appears most of the histology world disagrees with me but I stand by my post. If TRAP didn't work with formic acid in our hands, a major pharmaceutical treatment wouldn't be ready to help women with post-menopausal osteoporosis. Ray
----- Original Message ----- From: "Sarah Mack" <[email protected]> To: [email protected] Sent: Thursday, April 2, 2015 10:21:21 AM Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain In our hands we have never had TRAP success on Formic acid decalcified tissue. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ________________________________________ From: [email protected] [[email protected]] On Behalf Of [email protected] [[email protected]] Sent: Thursday, April 02, 2015 1:02 PM To: [email protected] Subject: Histonet Digest, Vol 137, Issue 3 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Allowable temperature range (Linda Prasad (SCHN)) 2. Positive Control for IF? (Paula Sicurello) 3. C3d? (Paula Sicurello) 4. Re: Positive Control for IF? (Mark Tarango) 5. Re: Positive Control for IF? (Jim Burchette) 6. RE: C3d? (Sebree Linda A) 7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt) 8. Question about Formic acid decal and TRAP stain (Debra Siena) 9. RE: Question about Formic acid decal and TRAP stain (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 1 Apr 2015 22:39:00 +0000 From: "Linda Prasad (SCHN)" <[email protected]> Subject: RE: [Histonet] Allowable temperature range To: "'Tim H'" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="utf-8" If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: [email protected] | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.au&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2k&e= Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: [email protected] Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 2 Date: Wed, 1 Apr 2015 16:12:22 -0700 From: Paula Sicurello <[email protected]> Subject: [Histonet] Positive Control for IF? To: HistoNet <[email protected]> Message-ID: <CAPSjddaNxLOoPhtQ0y4=ahu2qg7nnzgbhwfwomfvfyfngym...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering..... Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula :-) ------------------------------ Message: 3 Date: Wed, 1 Apr 2015 16:14:16 -0700 From: Paula Sicurello <[email protected]> Subject: [Histonet] C3d? To: HistoNet <[email protected]> Message-ID: <capsjddyaak+kz1mvw+a_e9ydarazgw9-fnusvvxucfca7k8...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ------------------------------ Message: 4 Date: Wed, 1 Apr 2015 16:16:54 -0700 From: Mark Tarango <[email protected]> Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello <[email protected]> Cc: HistoNet <[email protected]> Message-ID: <CACJwj1KEaQTqBsSp+tGznrxmEwbPmBuByXOHcajbeLpd=_3...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 It is April 1st, isn't it? On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello <[email protected]> wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > [email protected] > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > > ------------------------------ Message: 5 Date: Wed, 1 Apr 2015 20:16:29 -0500 From: Jim Burchette <[email protected]> Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello <[email protected]> Cc: HistoNet <[email protected]> Message-ID: <candy+medmbzpgwoba68xwaeoxomgtya5ts5b32malmdi0dw...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Paula, does your institution perform autopsies? If so, have the staff notify you when there is a lupus nephritis case. Have them send you fresh renal cortex. Cut up the tissue into small cubes and freeze for later use as IF control. Run your battery of Ig's, compliment and light chains. Most should have some reactivity. You might be able to use a rejected kidney for C4d if it isn't to necrotic. Transplant heart and liver can also be used for rejection antibodies. JB On Wednesday, April 1, 2015, Paula Sicurello <[email protected]> wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > [email protected] <javascript:;> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > > -- Jim Burchette "Fly Fishing Bum" *<'(((><* ------------------------------ Message: 6 Date: Thu, 2 Apr 2015 12:25:05 +0000 From: Sebree Linda A <[email protected]> Subject: RE: [Histonet] C3d? To: 'Paula Sicurello' <[email protected]>, HistoNet <[email protected]> Message-ID: <77dd817201982748bc67d7960f2f76af121...@uwhc-mbx12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="utf-8" Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ Message: 7 Date: Thu, 2 Apr 2015 14:13:49 +0000 From: Nancy Schmitt <[email protected]> Subject: [Histonet] interface between Bond and Cerner CoPath Plus To: "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 8 Date: Thu, 2 Apr 2015 16:00:25 +0000 From: Debra Siena <[email protected]> Subject: [Histonet] Question about Formic acid decal and TRAP stain To: "[email protected]" <[email protected]> Message-ID: <by2pr07mb1055c4721c256106d8593b3dc...@by2pr07mb105.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena [email protected] <mailto:[email protected]%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= > ------------------------------ Message: 9 Date: Thu, 2 Apr 2015 10:10:11 -0600 From: Elizabeth Chlipala <[email protected]> Subject: [Histonet] RE: Question about Formic acid decal and TRAP stain To: Debra Siena <[email protected]>, "[email protected]" <[email protected]> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17FFC@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__www.premierlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=F3th4LJ6QUaggfgVvpdB5XB0_s-ZkCp_YEJKag4d-Ic&e= March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Debra Siena Sent: Thursday, April 02, 2015 10:00 AM To: [email protected] Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena [email protected] <mailto:[email protected]%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= > _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= End of Histonet Digest, Vol 137, Issue 3 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
