No ice forms in fixed, paraffin embedded tissue blocks at usual temperatures
and length of chilling time and temperatures.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Mon, 20 Apr 2015 19:06:17 +0200
> From: [email protected]
> To: [email protected]
> Subject: Re: FW: [Histonet] prevent wrinkles when cutting
>
> Hi there,
>
> I´m curious about the soaking thing. We have never done it in our lab.
> Which is the purpose to do it?
>
> Than, after facing the blocks, we chill them in a cold plate so, if
> wanting to do the soaking , when should we? I guess before placing them
> on the cold plate, but that may cause a bit of ice formation?
>
> Thanks a lot for your help
>
> Julio
>
>
> On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote:
> > Rachel,
> > First off, are you chilling and soaking the blocks after you face them?
> > Do that and see if there is a difference.
> > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon
> > (5-6) sections that are good. Cut slowly but consistently.
> > What microtome are you using? Are you using disposable blades and are they
> > sharp? Don't expect them to cut well if you use the same blade to face the
> > blocks. If you aren't using disposables, get some! They will make your life
> > easier.
> > You might try to find a histotech at a local hospital lab who might be able
> > to give you a hands-on lesson.
> > Don't despair! We all sat down at our microtomes those first times and
> > suffered trying to get perfect sections. It takes practice. You might make
> > some blank blocks or blocks with tissue you can spare to practice your
> > cutting techniques. I used to do this with my students and it really helped
> > them.
> > Good luck!
> >
> > Andi G.
> > ________________________________________
> > From: [email protected]
> > [[email protected]] on behalf of Rachel M Gonzalez
> > [[email protected]]
> > Sent: Monday, April 20, 2015 9:07 AM
> > To: [email protected]
> > Subject: [Histonet] prevent wrinkles when cutting
> >
> > Hi
> >
> > Thursday was the first time I ever used a microtome.... I move to a lab
> > that does not have someone dedicated to cutting. I already miss her.
> >
> > I have no problems getting ribbons of 10-30 sections long but the pieces
> > are half the size of the original block. I am guessing they are wrinkling.
> > What am I doing wrong?
> >
> > Thanks
> > Rachel
> > Senior Scientist....
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>
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