You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane. For me I am using 80% Ethanol.
Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 7:23 PM To: Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fixation of frozen section What about the Absolute Methanol? Sent from my iPhone > On Apr 28, 2015, at 8:16 PM, Keyser Gerald T <gkey...@uwhealth.org> wrote: > > Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. > > Gerry > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil > Sent: Tuesday, April 28, 2015 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fixation of frozen section > > Hi histonet, > > Would like to query about which fixative do you prefer for fresh frozen section slide before H&E staining? > Thanks for your advices > Manahil > HTL/ ASCP > > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
