You should be filtering your Hematoxylin on a daily basis regardless of what
the manufactures says. We use to filter twice a day since we did a traditional
overnight run and then again in the afternoon for specimens that had been
microwave processed. So much tissue washes off in the solutions they should be
changed or filtered fairly regularly to try and prevent cross contamination on
the slides.
You can also try increasing your rinse times and see if that doesn't help as
well.
Thanks,
Tim
>
> Message: 1
> Date: Mon, 21 Sep 2015 15:14:39 -0500
> From: "Sandra Cheasty" <[email protected]>
> To: "Histonet ([email protected])"
> <[email protected]>
> Subject: [Histonet] Hematoxylin Precipitate
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello all,
>
> Has anyone using Richard Allen Hematoxylin-2 noticed an odd
> artifact on the slides after using the Hematoxylin for more than a few days
> on their stainer? We are seeing small spore or pollen-like blue dots here and
> there on the slides. It is not coming from the water bath or our water supply
> on the stainer. I used sterile gloves, opened a new case of slides, dipped
> them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI
> again, air-dried and coverslipped them, and the blue dots were there. The
> only way we got rid of the blue artifact was to use new RA Hematoxylin-2
> every 2-3 days, which is a bit expensive.
>
> Thanks for your input, and if you can recommend a different,
> reasonably priced hematoxylin, that would be awesome.
>
> Cheers,
>
> Sandy
>
>
>
> Sandra J. Cheasty, HT (ASCP)
>
> Histology & Necropsy Supervisor
>
> UW-Madison, School of Veterinary Medicine
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