Hi, There is a reason that every manual with an elastic procedure says that the stain should be made up fresh. My guess is that the company is trying to prolong the shelf life by removing or reducing either the iodine or ferric chloride. Doing this will prevent the hematoxylin from turning black. If it don't work on the machine, you should do it by hand. The results will be MUCH better. And this stain is not that difficult.
Cheers, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet