Does anyone know whether there is a difference, quantitatively, in Oil red O fat staining of tissue fixed in NBF prior to embedding in OCT vs. fresh frozen tissue embedded in OCT? I know that sections from fresh frozen tissues must be post-fixed in formalin before staining with Oil Red O. But is there any benefit to using this preparation over tissues that are fixed in formalin prior to OCT embedding (aside from sectioning/slide adhesion issues previously described)? Is the staining similar, or does one preparation give better staining results? Much gratitude for any advice you can give, Michele
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