I find the same as Caroline Miller, about 20-25% with formalin fixation and routine processing.
C On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet < histonet@lists.utsouthwestern.edu> wrote: > hi All, I have done some experiments in this area for mouse brains, and I > find that there is actually an expansion of tissue after formalin fixation > (around 10%), but then certainly a shrinkage to 100% dehydration agent of > about 20% from the original size. We found similar results with alcohol, > acetone and THF. > > This is manual fluid changes or a day per solution, with no vacuum, temp or > pressure. > > Happy to share the data if anyone is interested > > yours, > mills > > On Mon, Feb 22, 2016 at 1:10 PM, Terri Braud via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > LOL...Shrinkage...heh, heh. > > But seriously, there should be little to no gross shrinkage from formalin > > fixation and if the specimen is properly fixed, then there should be very > > little gross shrinkage as it is dehydrated. That is supposed to be the > > point! If someone is getting 30% shrinkage, there is something seriously > > wrong with their processing schedule. > > Sincerely, Terri > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > > > Today's Topics: > > 2. formalin and shrinkage (Gudrun Lang) > > > > > > Message: 2 > > Date: Mon, 22 Feb 2016 16:59:21 +0100 > > From: "Gudrun Lang" <gu.l...@gmx.at> > > Subject: [Histonet] formalin and shrinkage > > Hi! > > Today someone asked me about shrinkage caused by the fixation with > > formaldehyde specially on skin-biopsies. She spoke about shrinkage of > 30% > > percent. In my opinion shrinkage is mainly caused by the processing with > > dehydration and defatting. Formaldehyde renders the tissue harder but not > > strictly smaller. > > What is the opinion of the community? > > Gudrun > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet