Thank you for your help. This is encouraging. Helen
-----Original Message----- From: Glover, Kimberly [mailto:[email protected]] Sent: Tuesday, March 22, 2016 11:38 AM To: Helen Fedor <[email protected]> Cc: histonet <[email protected]> Subject: RE: Plastic coverslip Loosening Helen, Submerge the slides with coverslip loosely attached in fresh xylene to reactivate the film resin. Allow to sit in xylene for approximately 1 minute. Afterwards remove slide and press down on a paper towel. This action will allow the film to reattach to the slide while pressing out any excess xylene from the slide. As for the film (containing tissue) that has completed detached from the slide, place the film against a clean slide and place in a slide basket. Submerge slide in fresh xylene and proceed with same procedure above. Sometimes the issue of loosening or lifting of film is seen over time when the coverslipped slides are placed in temperature/humidity conditions that are contrary to the manufacturer's recommendations. Check packaging for storage conditions recommended. I hope this helps. Kimberly A. Glover Life Sciences Product Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 www.polysciences.com -----Original Message----- From: Helen Fedor [mailto:[email protected]] Sent: Tuesday, March 22, 2016 11:06 AM To: Glover, Kimberly Subject: RE: Plastic coverslip Loosening The film. -----Original Message----- From: Glover, Kimberly [mailto:[email protected]] Sent: Tuesday, March 22, 2016 10:25 AM To: Helen Fedor <[email protected]> Subject: RE: Plastic coverslip Loosening Are you using plastic coverslipping film or the plastic coverslips? Kimberly -----Original Message----- From: Helen Fedor via Histonet [mailto:[email protected]] Sent: Tuesday, March 22, 2016 9:45 AM To: HISTONET ([email protected]) Subject: [Histonet] Plastic coverslip Loosening Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer. Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip. Thanks in advance. Helen L. Fedor _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
