Valerie,
We have 4 DAKOs. They are work-horses and are a very "open" platform.
You can use all of your own reagents, nothing proprietary if you choose. This
makes them good for research and optimizing your own protocols. They are also
very consistent and reliable for conducting the same protocol repeatedly. Much
cheaper to own and operate than a Ventana. This is not a slam on Ventana.
Ventana strainers are quite amazing but way more expensive than a DAKO.
The Ventana is partially open, or not open, depending on how you look at it.
You can force it to use some of your own reagents but you always rely somewhat
on Ventana reagents. These stainers are mostly designed to run the same set of
protocols over and over again, consistently (e.g. hospital). They are also
useful when used by technicians who want to "set it and forget it". If someone
doesn't really know IHC, and the troubleshooting involved sometimes, then this
is the unit for you if you can afford it. There is also the high pressure
sales team that accompanies any interest in Ventana units. Don't underestimate
their tenacity, especially if you agree to have a trial machine placed in your
lab for testing. They will not want to remove it - an assumed sale, if you
will.
I am not on the payroll for anyone but Merck, lest anyone think I am
trying to influence a sale one way or another. These are my personal
experiences and opinions that I hope you will find useful.
Phil.
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486
215-652-9750
215-993-0383 (fax)
[email protected]
-----Original Message-----
From: Murphy, Valerie via Histonet [mailto:[email protected]]
Sent: Wednesday, May 04, 2016 11:52 AM
To: '[email protected]'
Subject: [Histonet] Automated IHC instrument
Hello Histonetters,
Our tissue core is interested in purchasing an IHC instrument. It would be
used for the more routine assays such as ER/PR, Her2, Ki67 etc.
Can anyone make a recommendation? Leica, Ventana, Dako?
Thank you,
Valerie Ratliff B.Sc HTL(ASCP)
Research Assistant
Department of Oncology
Karmanos Cancer Institute
4100 John R
Detroit, MI 48201
Telephone: (313) 576 8282
Fax: (313) 576 8306
E-mail:
[email protected]<https://webmail.karmanos.org/owa/redir.aspx?REF=3fWOArMaNAXm4nDSak3jq-gnqda3Gmrjo2JqVL-dzSB85MreM3TTCAFtYWlsdG86bXVycGh5dkBrYXJtYW5vcy5vcmc.>
Better treatments. Better outcomes.
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