As I see it, there are 3 main objections about using human saliva as an amylase
source.In order of importance they are:1- you will never know the actual
concentration of the amylase and this will produce reproducibility problems.2-
along with the saliva you will introduce bacteria that may end being stained
and can cause misdiagnoses.3- it is absolutely disgusting.René
On Thursday, May 5, 2016 2:26 PM, Bob Richmond via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.
He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.
Bob Richmond
Samurai Pathologist
Maryville TN
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