Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?"
Safranin O - the solution your microbiology people use for Gram staining works quite well - can be used to mark small pieces of tissue before processing. It stains more densely than eosin, and unlike eosin it isn't fluorescent. I worked in a lab that used this quite successfully. They placed the small specimens on those blue pads before marking them with a drop of dye, processing through xylene (or aliphatic substitute - I don't remember) on a truly ancient Fisher processor. Both they and I liked the results. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
