We just use a blunt butcher knife. It works great. Low tech, low cost!

Cate Hardy

Senior Technical Officer (Mon-Tue) | Veterinary Enterprises, Faculty of Science
Charles Sturt University
Boorooma Street
Locked Bag 588
Wagga Wagga, NSW 2678
Australia
Tel: 69334000
Email:  v...@csu.edu.au
Email: cha...@csu.edu.au
www.csu.edu.au



-----Original Message-----
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu]
Sent: Saturday, 23 July 2016 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 152, Issue 20

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Today's Topics:

   1. Unscribe (Fatima Formadi)
   2. block scrapers (Lauren Sweeney)
   3. Re: block scrapers (Jay Lundgren)
   4. Re: block scrapers (Cooper, Brian)
   5. Re: block scrapers (Elizabeth Chlipala)
   6. Re: Reticulin Stain (taylor alan)
   7. H&E staining on tape (Judi Ford)
   8. Re: block scrapers (J B)
   9. Re: block scrapers (Jennifer MacDonald)
  10. Re: Reticulin Stain (Jennifer MacDonald)
  11. Re: block scrapers (Rene J Buesa)
  12. Crystals in PAS (Amos Brooks)
  13. Re: block scrapers (Roberta Horner)
  14. Air Bubbles (Charles Riley)
  15. Re: block scrapers (Morken, Timothy)


----------------------------------------------------------------------

Message: 1
Date: Thu, 21 Jul 2016 12:17:50 -0600
From: Fatima Formadi <fatimaform...@gmail.com>
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Unscribe
Message-ID:
<CAJfbtJ=92kxbwnh8iwrqo1gez52fa_cefe82kfy0p_ykgtl...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hi,
I wish to unscribe from this mailing list immediately please.

Thank you

Regards,
Fatima


------------------------------

Message: 2
Date: Thu, 21 Jul 2016 18:46:53 +0000
From: Lauren Sweeney <lmari...@uga.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] block scrapers
Message-ID:
<sn1pr02mb1677c626a6dd65cb7016f0e2c5...@sn1pr02mb1677.namprd02.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

Hi all,

My lab is in need of some tools to scrap the paraffin off the edges of the 
blocks after embedding. Does anyone have any recommendations for me?


Thanks!!
L


------------------------------

Message: 3
Date: Thu, 21 Jul 2016 14:04:06 -0500
From: Jay Lundgren <jaylundg...@gmail.com>
To: Lauren Sweeney <lmari...@uga.edu>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<CANCZNuZBg4J4zffDkcivUtf4=ox+qraa8mga18qkvsjpqly...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Buy cheap paring knives at the dollar store and blunt the edges?

On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 4
Date: Thu, 21 Jul 2016 19:08:02 +0000
From: "Cooper, Brian" <bcoo...@chla.usc.edu>
To: "Jay Lundgren" <jaylundg...@gmail.com>, "Lauren Sweeney"
<lmari...@uga.edu>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<c12647ad4408834c8ab48fb0389c63e301a6cbb...@chlaexmbh01.la.ad.chla.org>

Content-Type: text/plain; charset=us-ascii

I bought everyone really old pocket knives from flea markets--basically the 
same thing as what Jay said.  For the most part, these old knives were already 
really dull and didn't require any assistance to dull them further.

Good luck.

Brian


-----Original Message-----
From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 12:04 PM
To: Lauren Sweeney
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] block scrapers

Buy cheap paring knives at the dollar store and blunt the edges?

On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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------------------------------

Message: 5
Date: Thu, 21 Jul 2016 13:09:13 -0600
From: Elizabeth Chlipala <l...@premierlab.com>
To: Lauren Sweeney <lmari...@uga.edu>,
"'histonet@lists.utsouthwestern.edu'
(histonet@lists.utsouthwestern.edu)"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<14E2C6176416974295479C64A11CB9AE02BED535B447@SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Lauren

I have always used a very dull steak knife (sharp ones are too sharp and will 
cut the cassette) mine is probably over 20 years old (now I'm dating myself).  
I'm old school so I still use that but the rest of the techs will use the para 
trimmer.  The para trimmer is more ergonomically friendly less repetitive than 
a knife -  here is the link to the website

https://www.thermofisher.com/order/catalog/product/B3120205

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block scrapers

Hi all,

My lab is in need of some tools to scrap the paraffin off the edges of the 
blocks after embedding. Does anyone have any recommendations for me?


Thanks!!
L
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 6
Date: Thu, 21 Jul 2016 20:30:31 +0100 (BST)
From: taylor alan <aj.tay...@blueyonder.co.uk>
To: Rene J Buesa <rjbu...@yahoo.com>, Anita Buchiane
<abuchi...@bmhvt.org>, Rene J Buesa via Histonet
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Reticulin Stain
Message-ID:
<846648922.1768040.1469129431802.javamail.open-xcha...@oxbe1.tb.ukmail.iss.as9143.net>

Content-Type: text/plain; charset=UTF-8

Hi All

Here in our lab we routinely hand stain reticulin sections. Our preferred 
choice is the Gordon and Sweet method. The technique is easy to perform, 
diffing out can be easily controlled via the staining microscope and a variety 
of counterstains can be used in addition to the usual Neutral Red.

We still prefer to prepare our own in-house solutions and reagents rather than 
purchase expensive commercial products. Old fashioned I know! But this is the 
common way still in many small, independent UK labs.

Histonet is such a valuable resource. I look forward to reading daily 
submissions and comments.

Alan Taylor

Microtechnical Services

Exeter. Devon. UK

>
>     On 21 July 2016 at 17:09 Rene J Buesa via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
>
>     Gomori's Ren?
>
>     On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
>
>     Does anyone out there still do the retic by hand?  If so, can you
> share which procedure you use?  Thanks
>
>
>
>
>     _______________________________________________________________
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>
>
>     _______________________________________________
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>     Histonet@lists.utsouthwestern.edu
>     http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 7
Date: Thu, 21 Jul 2016 19:31:17 +0000
From: Judi Ford <jf...@cytomx.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] H&E staining on tape
Message-ID:
<sn2pr0501mb1007bbbb09740b7028501e89d4...@sn2pr0501mb1007.namprd05.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

Hi everyone,
I have a colleague that has been asked to do H&E staining on a frozen specimen 
collected on tape. The tape has been attached to the slide using nail polish. 
Can't use the CryoJane system due to the light flash. Her question is "will the 
nail polish disintegrate during the immersion in alcohol and xylene?". Another 
question is will the stains attach to the tape and obscure the tissue?  If so, 
what to do with about that. She is also worried that the tape will fall off 
into the staining dish and get lost. I'm thinking maybe using a 6 well 
disposable tissue culture plate for the staining of the tape then reattach it 
to the slide and coverslip.
Any suggestions?
Thanks so much,
Judi


------------------------------

Message: 8
Date: Thu, 21 Jul 2016 19:32:48 +0000
From: J B <craiga...@gmail.com>
To: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<canyxu7dctn2_68scusrxp7bxqqhxz8srrmjefybj6tyjczq...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Yes, search paratrimmer you will love it. It melts excess paraffin off blocks.

JB

On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 9
Date: Thu, 21 Jul 2016 12:45:55 -0700
From: Jennifer MacDonald <jmacdon...@mtsac.edu>
To: J B <craiga...@gmail.com>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>,Lauren Sweeney <lmari...@uga.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<of74d0809b.b833609d-on88257ff7.006c930b-88257ff7.006c9...@mtsac.edu>
Content-Type: text/plain; charset="UTF-8"



------------------------------

Message: 10
Date: Thu, 21 Jul 2016 12:51:41 -0700
From: Jennifer MacDonald <jmacdon...@mtsac.edu>
To: Anita Buchiane <abuchi...@bmhvt.org>
Cc: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Reticulin Stain
Message-ID:
<of68e3cb71.85106924-on88257ff7.006d1a15-88257ff7.006d1...@mtsac.edu>
Content-Type: text/plain; charset="UTF-8"



------------------------------

Message: 11
Date: Thu, 21 Jul 2016 20:52:26 +0000 (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<1022675503.2796010.1469134346522.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Use a regular one blade pocket knife (as?I used to do).Ren?

    On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:


 Hi all,

My lab is in need of some tools to scrap the paraffin off the edges of the 
blocks after embedding. Does anyone have any recommendations for me?


Thanks!!
L
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 12
Date: Fri, 22 Jul 2016 04:52:37 -0400
From: Amos Brooks <amosbro...@gmail.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Crystals in PAS
Message-ID:
<cac95ki-qdcq-3g+5b+1scoorn+k5wteukn4lp7plko9ctwz...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hi,
   If you are having trouble with the instrument and the manufacturer isn't 
able to fix it, just hand stain them. PAS is really not a complicated stain. 
Please don't just accept underperforming equipment when we are all capable of 
so much more.

Amos


------------------------------

Message: 13
Date: Fri, 22 Jul 2016 14:14:48 +0000
From: Roberta Horner <r...@psu.edu>
To: "Histonet (histonet@lists.utsouthwestern.edu)"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID: <922f26cac06547eeb147719d6354a...@psu.edu>
Content-Type: text/plain; charset="iso-8859-1"

I use a single edge razor blade that I've opened boxes with and is dulled to 
just the right sharpness. And I wish people would quit throwing it away Roberta 
Horner Animal Diagnostic Lab Penn State


------------------------------

Message: 14
Date: Fri, 22 Jul 2016 10:42:50 -0400
From: Charles Riley <cri...@dpspa.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Air Bubbles
Message-ID:
<CAAQhB10DuzuOEenADLe=twoudm71kkt8sekm68ki9rqo+cx...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Has anyone encountered the following problem.

Tissue cut and is processed well. There is no distortion at a cellular level. 
However anywhere from 30 to 2 days later there are air bubbles under the 
coverslip. I know for certain there aren't even the slightest bit of air 
bubbles when coverslipped. I have a feeling it could still be a processing 
issue as I ran a few tests like coverslipping blank slides and tissue sections 
from a year ago when there was no issues. If anyone has any suggestions as to 
what the problem could be and potential solutions please get back to me as soon 
as possible as this is becoming a major issue for my lab.

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs


------------------------------

Message: 15
Date: Fri, 22 Jul 2016 15:29:58 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Lauren Sweeney <lmari...@uga.edu>
Cc: Histonet <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<761e2b5697f795489c8710bcc72141ff6fd6b...@ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"

Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from  a 
hardware store.

However, we moved away from scrapers and now use a heating block trimmer made 
for the purpose. Several vendors offer these.

http://www.newcomersupply.com/product/paraffin-wax-trimmer

We do all block trimming at a central location so it does not need to be done 
at the microtome.

It is very quick and does not damage the print on the block. That is especially 
important for barcoded blocks.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-----Original Message-----
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 11:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] block scrapers

Hi all,

My lab is in need of some tools to scrap the paraffin off the edges of the 
blocks after embedding. Does anyone have any recommendations for me?


Thanks!!
L
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Subject: Digest Footer

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------------------------------

End of Histonet Digest, Vol 152, Issue 20
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