We just use a blunt butcher knife. It works great. Low tech, low cost!
Cate Hardy Senior Technical Officer (Mon-Tue) | Veterinary Enterprises, Faculty of Science Charles Sturt University Boorooma Street Locked Bag 588 Wagga Wagga, NSW 2678 Australia Tel: 69334000 Email: v...@csu.edu.au Email: cha...@csu.edu.au www.csu.edu.au -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, 23 July 2016 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Unscribe (Fatima Formadi) 2. block scrapers (Lauren Sweeney) 3. Re: block scrapers (Jay Lundgren) 4. Re: block scrapers (Cooper, Brian) 5. Re: block scrapers (Elizabeth Chlipala) 6. Re: Reticulin Stain (taylor alan) 7. H&E staining on tape (Judi Ford) 8. Re: block scrapers (J B) 9. Re: block scrapers (Jennifer MacDonald) 10. Re: Reticulin Stain (Jennifer MacDonald) 11. Re: block scrapers (Rene J Buesa) 12. Crystals in PAS (Amos Brooks) 13. Re: block scrapers (Roberta Horner) 14. Air Bubbles (Charles Riley) 15. Re: block scrapers (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Jul 2016 12:17:50 -0600 From: Fatima Formadi <fatimaform...@gmail.com> To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Unscribe Message-ID: <CAJfbtJ=92kxbwnh8iwrqo1gez52fa_cefe82kfy0p_ykgtl...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi, I wish to unscribe from this mailing list immediately please. Thank you Regards, Fatima ------------------------------ Message: 2 Date: Thu, 21 Jul 2016 18:46:53 +0000 From: Lauren Sweeney <lmari...@uga.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] block scrapers Message-ID: <sn1pr02mb1677c626a6dd65cb7016f0e2c5...@sn1pr02mb1677.namprd02.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L ------------------------------ Message: 3 Date: Thu, 21 Jul 2016 14:04:06 -0500 From: Jay Lundgren <jaylundg...@gmail.com> To: Lauren Sweeney <lmari...@uga.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <CANCZNuZBg4J4zffDkcivUtf4=ox+qraa8mga18qkvsjpqly...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Thu, 21 Jul 2016 19:08:02 +0000 From: "Cooper, Brian" <bcoo...@chla.usc.edu> To: "Jay Lundgren" <jaylundg...@gmail.com>, "Lauren Sweeney" <lmari...@uga.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <c12647ad4408834c8ab48fb0389c63e301a6cbb...@chlaexmbh01.la.ad.chla.org> Content-Type: text/plain; charset=us-ascii I bought everyone really old pocket knives from flea markets--basically the same thing as what Jay said. For the most part, these old knives were already really dull and didn't require any assistance to dull them further. Good luck. Brian -----Original Message----- From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:04 PM To: Lauren Sweeney Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] block scrapers Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Thu, 21 Jul 2016 13:09:13 -0600 From: Elizabeth Chlipala <l...@premierlab.com> To: Lauren Sweeney <lmari...@uga.edu>, "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B447@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Lauren I have always used a very dull steak knife (sharp ones are too sharp and will cut the cassette) mine is probably over 20 years old (now I'm dating myself). I'm old school so I still use that but the rest of the techs will use the para trimmer. The para trimmer is more ergonomically friendly less repetitive than a knife - here is the link to the website https://www.thermofisher.com/order/catalog/product/B3120205 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 21 Jul 2016 20:30:31 +0100 (BST) From: taylor alan <aj.tay...@blueyonder.co.uk> To: Rene J Buesa <rjbu...@yahoo.com>, Anita Buchiane <abuchi...@bmhvt.org>, Rene J Buesa via Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Reticulin Stain Message-ID: <846648922.1768040.1469129431802.javamail.open-xcha...@oxbe1.tb.ukmail.iss.as9143.net> Content-Type: text/plain; charset=UTF-8 Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red. We still prefer to prepare our own in-house solutions and reagents rather than purchase expensive commercial products. Old fashioned I know! But this is the common way still in many small, independent UK labs. Histonet is such a valuable resource. I look forward to reading daily submissions and comments. Alan Taylor Microtechnical Services Exeter. Devon. UK > > On 21 July 2016 at 17:09 Rene J Buesa via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > > Gomori's Ren? > > On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > > Does anyone out there still do the retic by hand? If so, can you > share which procedure you use? Thanks > > > > > _______________________________________________________________ > > The information contained in, or attached to, this e-mail, may > contain confidential information and is intended solely for the use of > the individual or entity to whom it is addressed and may be subject to > legal privilege. If you have received this e-mail in error you should > notify the sender immediately by reply e-mail, delete the message from > your system and notify your system manager. Please do not copy it for > any purpose, or disclose its contents to any other person. The views > or opinions presented in this e-mail are solely those of the author > and do not necessarily represent those of the company. The recipient > should check this e-mail and any attachments for the presence of > viruses. The company accepts no liability for any damage caused, directly or > indirectly, by any virus transmitted in this email. > _______________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Thu, 21 Jul 2016 19:31:17 +0000 From: Judi Ford <jf...@cytomx.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] H&E staining on tape Message-ID: <sn2pr0501mb1007bbbb09740b7028501e89d4...@sn2pr0501mb1007.namprd05.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hi everyone, I have a colleague that has been asked to do H&E staining on a frozen specimen collected on tape. The tape has been attached to the slide using nail polish. Can't use the CryoJane system due to the light flash. Her question is "will the nail polish disintegrate during the immersion in alcohol and xylene?". Another question is will the stains attach to the tape and obscure the tissue? If so, what to do with about that. She is also worried that the tape will fall off into the staining dish and get lost. I'm thinking maybe using a 6 well disposable tissue culture plate for the staining of the tape then reattach it to the slide and coverslip. Any suggestions? Thanks so much, Judi ------------------------------ Message: 8 Date: Thu, 21 Jul 2016 19:32:48 +0000 From: J B <craiga...@gmail.com> To: Lauren Sweeney <lmari...@uga.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <canyxu7dctn2_68scusrxp7bxqqhxz8srrmjefybj6tyjczq...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Yes, search paratrimmer you will love it. It melts excess paraffin off blocks. JB On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Thu, 21 Jul 2016 12:45:55 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> To: J B <craiga...@gmail.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>,Lauren Sweeney <lmari...@uga.edu> Subject: Re: [Histonet] block scrapers Message-ID: <of74d0809b.b833609d-on88257ff7.006c930b-88257ff7.006c9...@mtsac.edu> Content-Type: text/plain; charset="UTF-8" ------------------------------ Message: 10 Date: Thu, 21 Jul 2016 12:51:41 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> To: Anita Buchiane <abuchi...@bmhvt.org> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Reticulin Stain Message-ID: <of68e3cb71.85106924-on88257ff7.006d1a15-88257ff7.006d1...@mtsac.edu> Content-Type: text/plain; charset="UTF-8" ------------------------------ Message: 11 Date: Thu, 21 Jul 2016 20:52:26 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> To: Lauren Sweeney <lmari...@uga.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <1022675503.2796010.1469134346522.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Use a regular one blade pocket knife (as?I used to do).Ren? On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 22 Jul 2016 04:52:37 -0400 From: Amos Brooks <amosbro...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Crystals in PAS Message-ID: <cac95ki-qdcq-3g+5b+1scoorn+k5wteukn4lp7plko9ctwz...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi, If you are having trouble with the instrument and the manufacturer isn't able to fix it, just hand stain them. PAS is really not a complicated stain. Please don't just accept underperforming equipment when we are all capable of so much more. Amos ------------------------------ Message: 13 Date: Fri, 22 Jul 2016 14:14:48 +0000 From: Roberta Horner <r...@psu.edu> To: "Histonet (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <922f26cac06547eeb147719d6354a...@psu.edu> Content-Type: text/plain; charset="iso-8859-1" I use a single edge razor blade that I've opened boxes with and is dulled to just the right sharpness. And I wish people would quit throwing it away Roberta Horner Animal Diagnostic Lab Penn State ------------------------------ Message: 14 Date: Fri, 22 Jul 2016 10:42:50 -0400 From: Charles Riley <cri...@dpspa.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air Bubbles Message-ID: <CAAQhB10DuzuOEenADLe=twoudm71kkt8sekm68ki9rqo+cx...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I have a feeling it could still be a processing issue as I ran a few tests like coverslipping blank slides and tissue sections from a year ago when there was no issues. If anyone has any suggestions as to what the problem could be and potential solutions please get back to me as soon as possible as this is becoming a major issue for my lab. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 15 Date: Fri, 22 Jul 2016 15:29:58 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: Lauren Sweeney <lmari...@uga.edu> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] block scrapers Message-ID: <761e2b5697f795489c8710bcc72141ff6fd6b...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a hardware store. However, we moved away from scrapers and now use a heating block trimmer made for the purpose. Several vendors offer these. http://www.newcomersupply.com/product/paraffin-wax-trimmer We do all block trimming at a central location so it does not need to be done at the microtome. It is very quick and does not damage the print on the block. That is especially important for barcoded blocks. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 20 ***************************************** Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. 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